Objective To investigate the relationship between the expression of long coding ＲNA FOXD2 adjacent opposite strand ＲNA 1 ( lncＲNA FOXD2-AS1) and miＲ-1913 in non-small cell lung cancer ( NSCLC) tissue and survival pe-riod． Methods A total of 100 NSCLC patients admitted to Binzhou Medical College Yantai Affiliated Hospital Cancer Center from November 2017 to November 2019 were regarded as the study subjects． Ｒeal-time fluorescence quantitative polymerase chain reaction ( qＲT-PCＲ) was applied to determine the expression levels of lncＲNA FOXD2-AS1 and miＲ-1913 in tissues; Pearson method was applied to analyze the correlation between lncＲNA FOXD2-AS1 and miＲ-1913 expression levels in NSCLC tissues; Kaplan-Meier method was applied to analyze the relationship between lncＲNA FOXD2-AS1，miＲ-1913 expression and prognosis in NSCLC tissues; Cox was applied to analyze the influencing factors of prognosis in NSCLC patients． Ｒesults Compared with the adjacent tissues，the expression level of lncＲNA FOXD2-AS1 in NSCLC tissues increased and the expression level of miＲ-1913 decreased ( t = 11． 439，17． 709，P ＜ 0． 001) ． Target Scan Human website predicts that there is a binding site between lncＲNA FOXD2-AS1 and miＲ-1913． The expression level of lncＲNA FOXD2-AS1 in NSCLC was negatively correlated with miＲ-1913 ( r = － 0． 406，P ＜ 0． 001) ． Compared with the survival subgroup，the expression level of lncＲNA FOXD2-AS1 in the death subgroup increased and the expression level of miＲ-1913 decreased ( t = 6． 973，6． 307，P ＜ 0． 001) ． The high expression rate of lncＲNA FOXD2-AS1 in NSCLC patients with TNM stage Ⅲ + Ⅳ，lymph node metastasis and low differentiation was higher than that in NSCLC patients with TNM stage Ⅰ + Ⅱ ( χ 2 = 5． 962，P = 0． 015) ， no lymph node metastasis ( χ 2 = 5． 104，P = 0． 024) and moderate /high differentiation( χ 2 = 6． 150，P = 0． 013) ． The low expression rate of miＲ-1913 in NSCLC patients with TNM stage Ⅲ + Ⅳ，lymph node metastasis and low differentiation was higher than that in NSCLC patients with TNM stage Ⅰ + Ⅱ ( χ 2 = 11． 457，P = 0． 001) ，no lymph node metastasis ( χ 2 = 7． 695， P = 0． 006) and moderate /high differentiation( χ 2 = 11． 349，P = 0． 001) ． The 3-year survival rate of NSCLC patients with high expression of lncＲNA FOXD2-AS1 was lower than that of patients with low expression of lncＲNA FOXD2-AS1 ( χ 2 = 7． 830，P = 0． 005) ，and that of patients with high expression of miＲ-1913 was higher than that of patients with low expression of miＲ-1913 ( χ 2 = 6． 125，P = 0． 013) ． Multivariate Cox analysis showed that high miＲ-1913 was a protective factor for the prognosis of NSCLC patients，the high level of lncＲNA FOXD2-AS1，TNM Ⅲ + Ⅳ stage，lymph node metastasis and low degree of differentiation are the risk factors for the prognosis of NSCLC patients［OＲ ( 95% CI) = 0． 864( 0． 774 － 0． 964) ，2． 544 ( 1． 481 － 4． 370) ，3． 647( 1． 614 － 8． 242) ，2． 544( 1． 481 － 4． 370) ，2． 986( 1． 361 － 6． 553) ］． Conclusion The expression level of lncＲNA FOXD2-AS1 increases and the expression level of miＲ-1913 decreases in NSCLC tissue，both of which are related to the prognostic survival period of patients．

Objective To investigate the expression and clinical significance of sirtiun 5 ( SIＲT5) and cysteine rich protein 1 ( CＲIP1) in hepatocellular carcinoma ( HCC) tissues． Methods A total of 150 HCC patients were selected for Hepatobiliary Surgery in Heping Hospital Affiliated to Changzhi Medical College from January 2018 to May 2020． The expressions of SIＲT5 and CＲIP1 in HCC tissues and adjacent tissues were detected by immunohistochemistry． The relationship between the expression of SIＲT5 and CＲIP1 and clinicopathological parameters of HCC patients was analyzed． According to the expression levels of SIＲT5 and CＲIP1 in HCC tissues，HCC patients were divided into positive /negative SIＲT5 and CＲIP1 expression groups． Kaplan-Meier method was used to plot the survival curve of HCC patients with positive /negative expression of SIＲT5 and CＲIP1． The factors influencing the prognosis of HCC patients were analyzed by Cox regression． Ｒesults Compared with para-cancerous tissues，HCC tissues showed decreased expression of SIＲT5 positivity and increased expression of CＲIP1 positivity ( χ 2 = 40． 991，42． 946，all P ＜ 0． 001) ． HCC tissues with single tumor，small tumor diameter，moderate to high differentiation，no vascular invasion，and TNM stage Ⅰ to Ⅱ，had increased SIＲT5 positive expression and decreased CＲIP1 positive expression，all of which were statistically significant( SIＲT5: χ 2 /P = 5． 179 /0． 023、6． 459 /0． 011、5． 899 / 0． 015、7． 402 /0． 007、5． 930 /0． 015; CＲIP1: χ 2 /P = 4． 275 /0． 039、5． 442 /0． 002、6． 459 /0． 011、6． 394 /0． 011、5． 655 /0． 017) ; At 3-year follow-up，the 3-year overall survival rate of 150 HCC patients was 56． 83% ( 79 /139) ． Kaplan-Meier survival curve analysis showed that the 3-year overall survival rate was higher in the SIＲT5-positive expression group than that in the SIＲT5-negative expression group，and the 3-year overall survival rate was higher in the CＲIP1-positive expression group than that in the CＲIP1-negative expression group ( Log-rank χ 2 = 7． 552，20． 942，P = 0． 006，＜ 0． 001) ． Multifactorial Cox regression analysis showed that multiple tumor numbers，tumor diameters ≥ 5cm，hypo-differentiation，vascular invasion， TNM stage Ⅲ，and CＲIP1 positivity were independent risk factors for death in HCC patients，and SIＲT5 positivity was an independent protective factor［OＲ ( 95% CI) = 2． 685 ( 1． 031 － 6． 999) ，2． 252 ( 1． 143 － 4． 439) ，4． 181 ( 1． 735 － 10． 076) ， 3． 945 ( 1． 653 － 9． 419) ，3． 485 ( 1． 492 － 8． 141) ，4． 540 ( 1． 641 － 12． 562) ，0． 207 ( 0． 085 － 0． 506) ］． Conclusion Low expression of SIＲT5 and high expression of CＲIP1 in HCC tissues are associated with tumor number，tumor diameter，degree of differentiation，vascular invasion，TNM stage and prognosis．

Objective To investigate the expression and clinical significance of Cullin4B E3 ubiquitin ligase ( CUL4B) and human transient receptor potential M2 ( TＲPM2) in pancreatic cancer． Methods Eighty-six patients with pancreatic cancer who were diagnosed and treated in author’s hospital from March 2019 to March 2020 were selected． Immunohistochemistry was used to detect the expression of CUL4B and TＲPM2 proteins in tissues． Ｒeal time fluorescence quantitative PCＲ was used to detect the expression of CUL4B and TＲPM2 mＲNA in tissues． Spearman rank correlation analysis was used to analyze the relationship between CUL4B and TＲPM2 protein expression． Kaplan-Meier curve analysis was used to analyze the impact of CUL4B and TＲPM2 expression on patient prognosis． Cox proportional hazards model was used to analyze the prognostic factors of pancreatic cancer． Ｒesults The positive rate of CUL4B and TＲPM2 protein and the relative expression of mＲNA in pancreatic cancer tissues were higher than those in adjacent tissues，and the difference was statistically significant ( χ 2 /t = 70． 245，60． 224，15． 741，10． 976，P ＜ 0． 001) ． The expression of CUL4B was positively correlated with TＲPM2 in pancreatic cancer ( r = 0． 720，P ＜ 0． 001) ． The positive rates of CUL4B and TＲPM2 protein in TNM stage Ⅱ B － Ⅲ，lymph node metastasis pancreatic cancer were higher than those in stage Ⅰ － Ⅱ A，and no lymph node metastasis ( χ 2 = 16． 511， 14． 834，15． 576，15． 007，P ＜ 0． 001) ． The 3-year overall survival rates of the CUL4B positive and negative groups were 9． 68% ( 6 /62) and 41． 67% ( 10 /24) ，respectively． The 3-year overall survival rates of the TＲPM2 positive and negative groups were 8． 33% ( 5 /60) and 42． 31% ( 11 /26) ，respectively． The 3-year cumulative survival rate of patients in the CUL4B positive group and TＲPM2 positive group was lower than that in the CUL4B negative group and TＲPM2 negative group，and the difference was statistically significant ( Log-Ｒank χ 2 = 9． 933，11． 102，P = 0． 002，0． 001) ． TNM stage， lymph node metastasis，CUL4B，TＲPM2 were independent factors affecting the prognosis of pancreatic cancer［HＲ( 95% CI) = 1． 781( 1． 199 － 2． 646) ，1． 962( 1． 172 － 3． 285) ，2． 482( 1． 445 － 4． 263) ，1． 733( 1． 223 － 2． 457) ］． Conclusion The expression of CUL4B and TＲPM2 in pancreatic cancer are elevated，which are related to the poor clinicopathological characteristics of pancreatic cancer and are prognostic tumor markers of pancreatic cancer．

Objective To explore the correlation between the expression of long non-coding ＲNA cancer susceptibility candidate 2 ( lncＲNA CASC2) ，microＲNA-532-3p ( miＲ-532-3p) in breast cancer ( BC) tissue and the survival of patients within 5 years after surgery． Methods A total of 127 BC patients admitted to Department of General Surgery，the Fifth People＇s Hospital of Datong City from January 2015 to June 2018 were selected，BC tissues and para-cancer normal tissues were collected during the operation，and the expressions of lncＲNA CASC2 and miＲ-532-3p in BC tissues and para-cancer normal tissues were detected by fluorescence quantitative PCＲ; the BC patients were followed up for 5 years after surgery，the survival and death of the patients within 5 years were recorded． The expressions of lncＲNA CASC2 and miＲ-532-3p in para-cancer normal tissues and BC tissues，the expression differences of lncＲNA CASC2 and miＲ-532-3p in BC tissues in different clinicopathological characteristics，the clinicopathological characteristics and the expressions of lncＲNA CASC2 and miＲ-532-3p in BC tissues of survival group and death group were compared． Analyze the correlation between the expression of lncＲNA CASC2 and miＲ-532-3p in BC tissue，the relationship between the expression of lncＲNA CASC2 and miＲ-532-3p in BC tissue and postoperative 5-year survival，the factors affecting postoperative 5-year survival of BC patients，and the predictive value of lncＲNA CASC2 and miＲ-532-3p for postoperative 5-year survival of BC patients． Ｒesults The expression level of lncＲNA CASC2 in BC tissues was significantly lower than that in para-cancer normal tissues，and the expression level of miＲ-532-3p in BC tissues was significantly higher than that in para-cancer normal tissues ( t /P = 38． 239 / ＜ 0． 001，49． 406 / ＜ 0． 001) ． The proportion of patients with tumor diameter ≥ 2 cm，TNM stage Ⅲ，low differentiation of tumors and lymph node metastasis in lncＲNA CASC2 low expression group were higher than in the high expression group，while the miＲ-532-3p low expression group was lower than that in the high expression group ( lncＲNA CASC2: χ 2 /P = 17． 361 / ＜ 0． 001，17． 052 / ＜ 0． 001， 14． 694 / ＜ 0． 001，13． 173 / ＜ 0． 001，miＲ-532-3p: χ 2 /P = 10． 733 /0． 001，9． 813 /0． 002，10． 134 /0． 001，7． 444 /0． 006) ． 127 BC patients were followed up for 5 years after surgery，without loss of follow-up，99 cases survived ( survival group) ，28 cases died ( death group) ． The proportion of patients with tumor diameter ≥ 2 cm，TNM stage Ⅲ，low differentiation of tumors，lymph node metastasis and miＲ-532-3p expression level in the death group were higher than in the survival group， while the lncＲNA CASC2 expression level in the death group was lower than in the survival group ［χ 2 ( t) /P = 5． 211 /0． 022， 27． 149 / ＜ 0． 001，27． 990 / ＜ 0． 001，4． 590 /0． 032，19． 155 / ＜ 0． 001，10． 818 / ＜ 0． 001］． LncＲNA CASC2 was negatively correlated with miＲ-532-3p expression in BC tissues ( r/P = － 0． 561 / ＜ 0． 001) ． The 5-year overall survival rate of BC patients after surgery in the lncＲNA CASC2 high expression group was 89． 23% ( 58 /65) higher than that in the lncＲNA CASC2 low expression group 66． 13% ( 41 /62) ( χ 2 /P = 9． 854 /0． 002) ; the 5-year overall survival rate of BC patients after surgery in the miＲ-532-3p high expression group was 65． 57% ( 40 /61) lower than that in the miＲ-532-3p low expression group 89． 39% ( 59 /66) ( χ 2 /P = 10． 466 /0． 001) ． Tumor diameter ≥ 2 cm，TNM stage Ⅲ，low differentiation of tumors，lymph node metastasis，lncＲNA CASC2 low expression，and miＲ-532-3p high expression were all independent risk factors affecting the survival of BC patients within 5 years after surgery［HＲ( 95% CI) = 2． 255( 1． 192 － 4． 263) ，2． 143 ( 1． 252 － 3． 666) ，3． 089 ( 1． 386 － 6． 887) ，2． 219 ( 1． 223 － 4． 026) ，2． 606 ( 1． 174 － 5． 788) ，2． 855 ( 1． 592 － 5． 120) ］． The area under curve ( AUC) of lncＲNA CASC2，miＲ-532-3p，their combined in predicting postoperative 5-year survival of BC patients was 0． 840，0． 852 and 0． 908，respectively，AUC predicted by the combination of the two methods was higher than that predicted by lncＲNA CASC2 and miＲ-532-3p separately ( Z/P = 2． 246 /0． 025，2． 033 /0． 042) ． Conclusion The expression of lncＲNA CASC2 is down-regulated and the expression of miＲ-532-3p is up-regulated in BC tissues，and the changes in BC patients who died within 5 years after surgery are more significant than those who survived． The expressions of both are correlated with clinicopathological characteristics，and are of high value in predicting the survival of BC patients within 5 years after surgery．

Objective To study the clinical value of micrornas 200c ( miＲ-200c) combined with MOＲC family CW type zinc finger protein 2 ( MOＲC2) detection in the assessment of ovarian cancer disease and prognosis． Methods A total of 103 patients with ovarian cancer ( ovarian cancer group) and 60 patients with benign ovarian diseases ( control group) diagnosed and treated in the Obstetrics and Gynecology Department of Ｒenmin Hospital of Wuhan University from January 2017 to December 2019 were selected as the study objects． Ｒeal-time fluorescence quantitative PCＲ was used to detect the expression of miＲ-200c and MOＲC2 and analyze their relationship with clinicopathological factors． Ｒeceiver Operating characteristic ( ＲOC) curve analysis of the efficacy of miＲ-200c combined with MOＲC2 in predicting 2-year poor prognosis of ovarian cancer． Multivariate Logistic regression analysis of risk factors of death in ovarian cancer patients． Kaplan-Meier method was used to analyze the relationship between miＲ-200c and MOＲC2 expression and survival． Ｒesults The expressions of miＲ-200c and MOＲC2 in tumor tissues of ovarian cancer group were higher than those in adjacent tissues and control group ( F =1 926． 617，1 832． 415，P ＜0． 001) ． The ex-pressions of miＲ-200c and MOＲC2 were higher in patients with low differentiation，malignant ascites，maximum tumor diameter ≥ 5 cm，lymph node metastasis，distant metastasis，and stage Ⅲ to Ⅳ ovarian cancer than those with medium-high differentiation，no malignant ascites，and maximum tumor diameter ＜5 cm，no lymph node metastasis，no distant metastasis and stageⅠtoⅡpatients ( t =14． 067，12． 451，12． 871，11． 523，16． 298，18． 351，11． 745，10． 893，10． 462，9． 893，14． 617，13． 269，P ＜ 0． 001) ． The AUC of miＲ-200c，MOＲC2 and their combination in predicting 2-year poor prognosis of ovarian cancer patients was 0． 786，0． 792 and 0． 901，respectively，and the combined efficacy of miＲ-200C was superior to that of miＲ-200C and MOＲC2 alone ( Z = 8． 239，8． 025，P ＜ 0． 001) ． miＲ-200c≥0． 78，MOＲC2≥0． 65，poorly differentiated，malignant ascites，maximum tumor diameter ≥5 cm，lymph node metastasis，distant metastasis，and FIGO stage Ⅲ to Ⅳ were independent risk factors for ovarian cancer death ［OＲ( 95% CI) = 5． 323 ( 1． 812 － 8． 834) ，4． 802 ( 1． 141 － 8． 463) ，2． 065 ( 1． 068 － 3． 062) ， 2． 252 ( 1． 145 － 3． 359) ，2． 140 ( 1． 216 － 3． 064) ，2． 012 ( 1． 005 － 3． 019) ，2． 522 ( 1． 625 － 3． 419) ，2． 186 ( 1． 134 － 3． 238) ］． The median survival time of ovarian cancer patients with miＲ-200c≥0． 78 and MOＲC2≥0． 65 was significantly lower than that of other patients ( miＲ-200c ＜ 0． 78 or MOＲC2 ＜ 0． 65) ( Log-Ｒank χ 2 = 16． 371，P ＜ 0． 05) ． Conclusion The expression of miＲ-200c and MOＲC2 is significantly increased in patients with ovarian cancer，which has important clinical value in the assessment of ovarian cancer disease and prognosis． The combined detection of miＲ-200C and MOＲC2 can significantly improve the sensitivity and specificity in predicting the poor prognosis of ovarian cancer．