Objective To investigate the expression and clinical significance of protein arginine methyltransferase 5 ( PＲMT5) and cyclin dependent kinase inhibitor 2B ( CDKN2B) in cervical cancer． Methods The clinical data of 88 cervical cancer patients who treated in the Department of Obstetrics and Gynecology，Gulou Hospital Affiliated to Nanjing University School of Medicine from March 2019 to March 2020 were collected． Immunohistochemical method was used to detect the expression of PＲMT5 and CDKN2B in cervical cancer and adjacent tissues． The correlation between PＲMT5 and CDKN2B expression was analyzed using Spearman correlation analysis． The differences of PＲMT5，CDKN2B in cervical cancer tissues with different clinical characteristics were compared． Kaplan-Meier curve was used to evaluate the impact of PＲMT5 and CDKN2B expression on the progression free survival prognosis of cervical cancer patients． Multivariate Cox regression analysis was used to analyze factors influencing the progression free survival prognosis of cervical cancer patients． Ｒesults The positive rate of PＲMT5 protein in cancer tissue was 70． 45% ( 62 /88) ，which was higher than 6． 82% ( 6 /88) in adjacent tissues ( χ 2 = 75． 155，P = ＜ 0． 001) ． The positive rate of CDKN2B in cervical cancer tissue was 22． 73% ( 20 /88) ，lower than 79． 55% ( 71 /88) in adjacent tissues ( χ 2 = 75． 336，P ＜ 0． 001) ． There was a significant negative correlation between PＲMT5 and CDKN2B in cervical cancer ( r = － 0． 734，P ＜ 0． 001) ． The positive rates of PＲMT5 and CDKN2B in cervical cancer tissues with different FIGO stages and lymph node metastasis were statistically significant ( P ＜ 0． 05) ． The cumulative 3-year progression free survival rates of the PRMT5 positive and negative groups were 74． 19% ( 46 /62) and 92． 31% ( 24 /26) ，respectively，the cumulative 3-year progression free survival rate of the PＲMT5 positive group was lower than that of the PＲMT5 negative group ( Log Ｒank χ 2 = 4． 386，P = 0． 017) ． The 3-year progression free survival rates of the CDKN2B negative and positive groups were 75． 00% ( 51 / 68) and 95． 00% ( 19 /20) ，respectively，the cumulative 3-year progression free survival rate of the CDKN2B negative group was lower than that of the CDKN2B positive group，and the difference was statistically significant ( Log Ｒank χ 2 = 4． 423，P = 0． 012) ． FIGO staging Ⅰ B2 to Ⅱ A，combined with lymph node metastasis，positive PＲMT5，and negative CDKN2B were independent risk factors affecting the progression free prognosis of cervical cancer patients［OＲ( 95% CI) = 1． 407 ( 1． 159 － 1． 696) ，1． 464 ( 1． 201 － 1． 784) ，1． 614 ( 1． 189 － 2． 192) ，1． 595 ( 1． 191 － 2． 136) ］． Conclusion The increased expression of PＲMT5 and the decreased expression of CDKN2B in cervical cancer tissue are related to the adverse clinical pathological characteristics of cervical cancer patients，and are biomarkers for evaluating the prognosis of cervical cancer．

Objective To investigate the expression significance of serum long non-coding ribonucleic acid ( LncＲNA) FAF and inter alpha trypsin inhibitor heavy chain 4 ( ITIH4) in patients with chronic heart failure ( CHF) and their prognostic value． Methods A total of 187 patients with CHF admitted to the Department of Emergency，the Second Affiliated Hospital of Anhui Medical University from January 2020 to January 2022 were selected as CHF group，and then divided into Grade Ⅱ subgroup ( 65 cases) ，grade Ⅲ subgroup ( 77 cases) ，and grade Ⅳ subgroup ( 45 cases) according to NYHA cardiac function classification． In the same period，103 healthy volunteers were recruited as control group． Serum LncＲNA FAF expression and ITIH4 level were detected，and the incidence of major adverse cardiovascular events ( MACE) during follow-up was analyzed for 12 months after discharge． Multivariate Logistic regression analysis was conducted to analyze the factors affecting the occurrence of MACE in CHF patients，and the value of LncＲNA FAF and ITIH4 in predicting the occurrence of MACE in CHF patients was analyzed by receiver operating characteristic ( ＲOC) curve． Ｒesults Serum LncＲNA FAF expression and ITIH4 level in CHF group were lower than those in control group ( t /P = 24． 469 / ＜ 0． 001，35． 196 / ＜ 0． 001) ． Serum LncＲNA FAF expression and ITIH4 level in grade Ⅳ group were lower than those in grade Ⅲ and grade Ⅱ groups ( F/P = 91． 653 / ＜ 0． 001，102．345 / ＜ 0． 001) ． Serum LncＲNA FAF expression and ITIH4 level in MACE group were lower than those in non-MACE group ( t /P = 13． 556 / ＜ 0． 001，6． 293 / ＜ 0． 001) ． NYHA grade Ⅳ and high level of NT-pro BNP were risk factors for MACE in CHF patients，high expression of LncＲNA FAF and high level of ITIH4 were protective factors［OＲ( 95% CI) = 4． 627 ( 2． 245 － 9． 538) ，2． 284 ( 1． 505 － 3． 468) ，0． 599 ( 0． 425 － 0． 844) ，0． 666 ( 0． 478 － 0． 928) ］． The area under the curve of LncＲNA FAF，ITIH4 and combined detection in predicting the occurrence of MACE in CHF patients was 0． 796，0． 801 and 0． 896，and the area under the curve of combined prediction was higher than that of single prediction ( Z = 2． 453，2． 404，all P ＜ 0． 001) ． Conclusion Serum LncＲNA FAF expression and ITIH4 level are decreased in CHF patients，and are associated with poor prognosis． Combining LncＲNA FAF and ITIH4 can predict the risk of poor prognosis in CHF patients．

Objective To investigate the levels of serum Salusin-α and Salusin-β in patients with coronary slow flow ( CSF) and their predictive value for CSF． Methods Clinical data of 108 patients who underwent coronary angiography ( CAG) examination in the Department of Cardiology，Inner Mongolia Autonomous Ｒegion People＇s Hospital from June 2019 to June 2022 and showed no obvious stenosis of the coronary artery were selected． According to the blood flow classification of the myocardial infarction thrombolysis test ( TIMI) ，they were divided into CSF group ( TIMI grade 2 and below，66 cases) and control group ( TIMI grade 3，42 cases) ． Enzyme-linked immunosorbent assay was used to measure the serum levels of Salusin-α and Salusin-β． Pearson linear correlation analysis was used to analyze the correlation between serum Salusin-α，Salusin-β levels and clinical indicators． Multivariate Logistic regression analysis was used to analyze the risk factors for CSF． Ｒeceiver operating characteristic curve was used to analyze the predictive value of serum Salusin-α，Salusin-β and their combination for CSF． Ｒesults Serum Salusin-β， smoking history，serum uric acid，left anterior descending artery ( LAD) ，left circumflex artery ( LCX) ，right coronary artery ( ＲCA) frame count and average frame count of three coronary arteries in CSF group were significantly higher than those in control group ( χ 2 /t/P =16． 702/ ＜0． 001，7． 013/0． 008，7． 917/ ＜0． 001，12． 074/ ＜0． 001，9． 344/ ＜0． 001，13． 627/ ＜0． 001，10． 476/ ＜ 0． 001) ，while serum albumin and serum Salusin-α levels were lower than those of the control group ( t/P = 8． 084/ ＜ 0． 001，19． 189/ ＜0． 001) ． Serum Salusin-α was negatively correlated with serum uric acid，LAD frame count，LCX frame count，ＲCA frame count and average frame count ( r/P = －0． 708/ ＜0． 001，－0． 769/ ＜0． 001，－0． 662/ ＜0． 001，－0． 635/ ＜0． 001，－0． 708/ ＜0． 001) and positively correlated with serum albumin ( r/P =0． 667/ ＜0． 001) ． Serum Salusin-β was positively correlated with serum uric acid，LAD frame count，LCX frame count，ＲCA frame count and average frame count ( r/P =0． 748/ ＜0． 001，0． 676/ ＜0． 001， 0． 753/ ＜0． 001，0． 642/ ＜ 0． 001，0． 776/ ＜ 0． 001) ，and negatively correlated with serum albumin ( r/P = － 0． 589 / ＜ 0． 001) ． Smoking history and Salusin-β were independent risk factors for CSF，while serum albumin and Salusin-α were protective factors［OＲ ( 95% CI) = 1． 464 ( 1． 146 － 1． 870) ，1． 536 ( 0． 928 － 2． 548) ，0． 819 ( 0． 710 － 0． 942) ，0． 715 ( 0． 582 － 0． 878) ，P ＜ 0． 05］． The AUC of serum Salusin-α，Salusin-β，and their combination for predicting CSF was 0． 840，0． 782，and 0． 896，respectively，and the combination of the two was better than each of them alone ( Z = 4． 561，4． 103，P = 0． 005，＜ 0． 001) ． Conclusion Serum Salusin-α is decreased and Salusin-β is increased in patients with CSF，which are related to the severity of CSF． The combination of serum Salusin-α and Salusin-β has a high predictive value for CSF．

Objective To investigate the relationship between the expression of serum inter alpha trypsin inhibitor heavy chain 4 ( ITIH4) ，myeloid cell leukemia sequence 1 ( MCL-1) and disease severity and prognosis in patients with acute ischemic stroke ( AIS) ． Methods A total of 128 patients diagnosed and treated with AIS in the Department of Neurology of Henan University of Science and Technology Affiliated Yellow Ｒiver Hospital from July 2019 to July 2022 as AIS group． According to the National Institutes of Health Stroke Scale ( NIHSS) score at admission，the patients were divided into mild subgroup ( NIHSS ＜ 6 points，n = 42) ，moderate subgroup ( 6 points ≤ NIHSS ＜ 14 points，n = 52) ，and severe subgroup ( NIHSS ≥ 14 points，n = 34) ． According to the modified Ｒankins score of AIS patients at 3 months of discharge，they were dividednto poor prognosis subgroup ( score ＞ 2 points，30 cases) and good prognosis subgroup ( score ≤ 2 points，98 cases) ． A total of 70 healthy individuals who underwent physical examinations in hospitals during the same period were selected as the healthy group． Enzyme linked immunosorbent assay was used to detect serum levels of ITIH4 and MCL-1． Pearson correlation analysis was conducted to investigate the correlation between serum levels of ITIH4 and MCL-1 with the severity and prognosis of the disease; Multivariate Logistic regression analysis was used to identify the factors that affect the prognosis of AIS patients; The predictive value of serum ITIH4 and MCL-1 for the prognosis of AIS patients was analyzed by receiver operating characteristic curves． Ｒesults The serum levels of ITIH4 and MCL-1 in AIS patients were lower than those in the healthy group ( t /P = 43． 211 / ＜ 0． 001，43． 191 / ＜ 0． 001) ． The more severe the illness，the lower the serum levels of ITIH4 and MCL-1 in AIS patients ( F/P = 107． 796 / ＜ 0． 001，297． 976 / ＜ 0． 001) ． The infarction area and 24-hour NIHSS score of the poor prognosis group were higher than those of the good prognosis group ( t /P = 9． 637 / ＜ 0． 001，9． 752 / ＜ 0． 001) ，while serum ITIH4 and MCL-1 levels，3-month Mini Intelligence State Scale ( MMSE) score and Montreal Cognitive Assessment Scale ( MoCA) score were lower than those of the good prognosis subgroup，with statistically significant differences ( t /P = 26． 723 / ＜ 0． 001，11． 709 / ＜ 0． 001，13． 674 / ＜ 0． 001，10． 782 / ＜ 0． 001) ． The serum ITIH4 and MCL-1 levels in AIS patients were negatively correlated with infarct size and 24-hour NIHSS score( r/P = － 0． 705 / ＜ 0． 001，－ 0． 685 / ＜ 0． 001，－ 0． 761 / ＜ 0． 001，－ 0． 619 / ＜ 0． 001) ，while positively correlated with MMSE score and MoCA score at 3 months after discharge( r/P = 0． 656 / ＜ 0． 001，0． 632 / ＜ 0． 001，0． 751 / ＜ 0． 001，0． 789 / ＜ 0． 001) ． High MMSE score and high MoCA score after discharge were independent protective factors affecting poor prognosis in AIS patients ［OＲ( 95% CI) = 0． 622 ( 0． 446 － 0． 868) ，0． 606 ( 0． 427 － 0． 861) ］，while low serum ITIH4，low serum MCL-1，large infarct size and high 24-hour NIHSS score were risk factors［OＲ ( 95% CI) = 1． 467 ( 1． 150 － 1． 870) ，1． 415 ( 1． 094 － 1． 829) ，1． 605 ( 1． 168 － 2． 205) ，1． 765 ( 1． 233 － 2． 526) ］． The AUC of serum ITIH4，MCL-1，and their combined prediction for poor prognosis in AIS were 0． 811，0． 835，and 0． 923，respectively． The AUC of the two combined predictions for poor prognosis in AIS was greater than that of single indicator，and the difference was statistically significant ( Z = 4． 258，4． 119，all P ＜ 0． 001) ． Conclusion The expression of serum ITIH4 and MCL-1 in AIS patients is downregulated，and their expression levels are related to the severity of the disease． The combination of the two has high predictive value for the prognosis of AIS patients．

Objective To analyze the expression of serum osteocalcin and deacetylase 6 ( SIＲT6) in patients with acute ischemic stroke ( AIS) and their evaluation value for clinical prognosis． Methods Ninety AIS patients ( AIS group) who treated in the Department of Neurology of People＇s Hospital Banan District from February 2019 to February 2021 were collected，90 patients with hypertension or diabetes treated at the same time were taken as the disease control group，and 90 healthy individuals were selected as the control group． Following up for 3 months，AIS patients were divided into a poor prognosis group ( n = 24) and a good prognosis group ( n = 66) based on their prognosis． Enzyme-linked immunosorbent assay was used to detect serum osteocalcin and SIＲT6 levels． Multivariate Logistic regression analysis was used to analyze factors affecting poor prognosis in AIS patients． The evaluation value of various indicators in evaluating the adverse prognosis of AIS patients through receiver operating curve analysis． Ｒesults Compared with the healthy control group，the serum levels of osteocalcin and SIＲT6 decreased in the AIS group ( t /P = 25． 013 / ＜ 0． 001，27． 571 / ＜ 0． 001) ． Compared with the non AIS group，the AIS group showed a significant decrease ( t /P = 31． 808 / ＜ 0． 001，36． 440 / ＜ 0． 001) ，while there was no statistically significant difference between the healthy control group and the non AIS group ( t /P = 2． 559 /0． 152，3． 113 /0． 067) ． The serum os-teocalcin and SIＲT6 levels in AIS patients in the severe，moderate，and mild subgroups decreased sequentially ( F/P = 15． 234 / ＜ 0． 001，30． 388 / ＜ 0． 001) ． Compared with the subgroup with good prognosis，AIS patients in the subgroup with poor prognosis have larger infarct size，higher NIHSS score，and lower serum osteocalcin and SIＲT6 ( χ 2 /t = 8． 511，22． 826， 3． 592，3． 729，all P ＜ 0． 001) ． Large infarct size and high NIHSS score are independent risk factors for poor prognosis in AIS patients ［OＲ( 95% CI) = 1． 397 ( 1． 141 － 1． 709) ，1． 363 ( 1． 076 － 1． 728) ］． Low serum osteocalcin and SIＲT6 are independent protective factors affecting the poor prognosis of AIS patients ［OＲ ( 95% CI) = 0． 770 ( 0． 610 － 0． 973) ，0． 709 ( 0． 549 － 0． 915) ］． The AUC of serum osteocalcin，SIＲT6，and their combination for adverse prognosis in AIS patients were 0． 782，0． 796，and 0． 886，respectively． The combined AUC of the two items was greater than their individual predictive efficacy ( Z = 4． 526，4． 209，P ＜ 0． 001) ． Conclusion The decrease of serum osteocalcin and SIＲT6 in AIS patients are related to the severity and prognosis of AIS，and the combination of the two can improve the predictive value of poor prognosis。