Objective To analyze the expression of microribonucleic acid-330-5p(miR-330-5p) and polypyrimidine region binding protein 1(PTBP1) in colorectal cancer tissue and their relationship with pathological parameters and prognosis. Methods One hundred and one patients with colorectal cancer admitted to the Anorectal Department of Nantong Traditional Chinese Medicine Hospital from January 2018 to May 2020 were selected. Part of the cancer tissue and its adjacent tissues were collected during surgery, and real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of miR-330-5p and PTBP1 mRNA. Predict the binding sites of miR-330-5p and PTBP1 through the Targetscan database, and analyze the differences of miR-330-5p and PTBP1 mRNA in different clinical and pathological parameters of colorectal cancer tissue; Draw its survival curve using the K-M method; Multivariate Cox regression analysis of prognostic factors in colorectal cancer patients. Results Compared with adjacent tissues, the expression of miR-330-5p in colorectal cancer tissue decreased, while the expression of PTBP1 mRNA increased(t/P=24.000/<0.001, 19.233/<0.001). MiR-330-5p has a binding site with PTBP1, and their expression in colorectal cancer tissue is negatively correlated(r/P=-0.679/<0.001). Compared with low differentiation, TNM stage Ⅲ, and lymph node metastasis, colorectal cancer tissues with medium to high differentiation, TNM stage Ⅰ-Ⅱ, and no lymph node metastasis showed an increase in miR-330-5p and a decrease in PTBP1 mRNA expression(t/P=2.490/0.014, 2.479/0.015, 2.837/0.006, 2.953/0.004, 3.319/0.001, 3.307/0.001). The overall 3-year survival rate of 101 patients with colorectal cancer was 83.17%(84/101). The 3-year overall survival rates of the miR-330-5p high expression group and the PTBP1 mRNA low expression group were higher than those of the miR-330-5p low expression group and the PTBP1 mRNA high expression group, respectively(χ2/P=6.466/0.011, 11.697/0.001). The independent risk factors for mortality in colorectal cancer patients are low differentiation, TNM stage Ⅲ, lymph node metastasis, and PTBP1 mRNA ≥ 1.50. The independent protective factors are miR-330-5p ≥ 0.57 [OR(95%CI)=3.642(1.278-10.381), 3.817(1.375-10.598), 4.013(1.418-11.351), 2.684(1.025-7.029), 0.338(0.129-0.890)].Conclusion Low expression of miR-330-5p and high expression of PTBP1 mRNA in colorectal cancer tissue are associated with differentiation degree, TNM staging, lymph node metastasis, and prognosis.

Objective To analyze the expression significance of lysine specific demethylase 1(LSD1) and piezoelectric mechanosensitive ion channel component 1(PIEZO1) in oral cancer and their relationship with prognosis. Methods One hundred and thirteen patients with oral cancer admitted to the Department of Stomatology at the 920th Hospital of the Joint Logistics Support Force from January 2013 to March 2018 were selected. Immunohistochemical methods were used to detect the expression of LSD1 and PIEZO1 in the intraoperative and adjacent oral cancer tissues; Analyze the relationship between the expression of LSD1 and PIEZO1 in oral cancer tissue and clinical pathological characteristics; According to the expression of LSD1 and PIEZO1 in oral cancer tissue, it is divided into LSD1 positive expression group, PIEZO1 positive expression group, LSD1 negative expression group, and PIEZO1 negative expression group; The Kaplan Meier method was used to plot the survival curves of oral cancer patients with positive/negative expression of LSD1 and PIEZO1, and Cox regression analysis was used to analyze the factors affecting the prognosis of oral cancer patients.Results Compared with adjacent tissues, the positive expression rates of LSD1 and PIEZO1 are increased in oral cancer tissues(χ2/P=47.684/<0.001, 43.929/<0.001). lsd1="" and="" piezo1="" have="" increased="" positive="" expression="" rates="" in="" patients="" with="" low="" infiltration="" depth="">5 mm, TNM stage Ⅲ, and lymph node metastasis(LSD1: χ2/P=5.815/0.016, 6.669/0.010, 8.145/0.004, 10.879/0.001, PIEZO1: χ2/P=6.136/0.013, 5.796/0.016, 6.771/0.009, 8.116/0.004). The 5-year overall survival rate of 113 oral cancer patients was 56.64%(64/113). Kaplan Meier survival curve analysis showed that the overall 5-year survival rates of LSD1 positive expression group and PIEZO1 positive expression group were lower than those of LSD1 negative expression group and PIEZO1 negative expression group, respectively(χ2/P=12.097/0.001, 9.795/0.002). Low differentiation, infiltration depth>5 mm, TNM stage Ⅲ, lymph node metastasis, and positive expression of LSD1 and PIEZO1 are independent risk factors affecting the prognosis of oral cancer patients [OR(95%CI)=3.131(1.129-8.679), 4.011(1.426-11.283), 5.100(1.274-20.417), 8.357(1.800-38.795), 3.623(1.059-12.395), 3.454(1.191-10.019)].Conclusions The high expression of LSD1 and PIEZO1 in oral cancer tissue is related to differentiation degree, infiltration depth, TNM staging, lymph node metastasis, and prognosis, and may become biomarkers for evaluating the prognosis of oral cancer patients.

Objective To construct a risk prediction model for immune related genes(IRGs) to predict the prognosis of oral squamous cell carcinoma(OSCC) patients. Methods Applying bioinformatics technology to analyze transcriptome sequencing data of OSCC and identify differentially expressed IRGs(DEIRGs). Construct a risk prediction model for DEIRGs through Cox regression analysis and evaluate its predictive ability. Analyze the correlation between the model and clinical pathology and immune cell infiltration. Results By comparing OSCC and normal samples, a total of 3634 differentially expressed genes were identified, including 330 DEIRGs(FDR<0.05, logfc="">1). Univariate Cox regression analysis identified 20 DEIRGs related to prognosis(P<0.05), while multivariate Cox regression analysis identified 15 DEIRGs for constructing a risk prediction model. This model can serve as an independent prognostic factor for OSCC patients(P<0.001), with high accuracy in predicting patient prognosis(AUC=0.732), and is closely related to clinical staging(t=-3.484, P<0.001), B cells(Cor=-0.180, P=0.002), and CD4+T cells(Cor=-0.127, P=0.026). Conclusion A risk prediction model based on 15 prognostic related DEIRGs can effectively predict the prognosis of OSCC patients and help clinicians choose personalized treatment strategies for OSCC patients with different risks.

Objective To investigate the relationship between serum levels of microribonucleic acid 22-3p(miR-22-3p) and NOD like receptor heat protein domain related protein 3(NLRP3) in patients with acute cerebral infarction(ACI), inflammatory factors, and poor prognosis. Methods One hundred and six patients with ACI admitted to the Neurology Department of the Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from January 2021 to December 2022 were selected as the ACI group. According to the prognosis, they were divided into a subgroup of 37 patients with poor prognosis and a subgroup of 69 patients with good prognosis. Additionally, 60 healthy individuals who underwent physical examinations during the same period were selected as the healthy control group. Real time fluorescence quantitative polymerase chain reaction was used to detect serum miR-22-3p levels. Enzyme-linked immunosorbent assay for detecting serum NLRP3 and inflammatory factors [ interleukin(IL-1) ] β, IL-18, Tumor Necrosis Factor-α(TNF-α)]. Pearson was used to analyze the relationship between serum miR-22-3p and NLRP3 with IL-1β, IL-18 and TNF-α in ACI patients. Analyze the influencing factors of poor prognosis in ACI patients and draw subject operating characteristic(ROC) curves for two indicators. Results Compared with the healthy control group, the serum levels of miR-22-3p in the ACI group decreased, and the levels of NLRP3, IL-1β, IL-18 and TNF-α increased(t/P=18.698/<0.001, 27.091/<0.001, 30.154/<0.001, 35.104/<0.001, 39.834/<0.001). Pearson correlation analysis showed that serum miR-22-3p was negatively correlated with the levels of NLRP3, IL-1β, IL-18 and TNF-α in ACI patients(r/P=-0.733/<0.001,-0.719/<0.001,-0.683/<0.001,-0.680/<0.001), and serum NLRP3 was positively correlated with the levels of IL-1β, IL-18 and TNF-α(r/P=0.716/<0.001、0.715/<0.001、0.707/<0.001). Multivariate Logistic regression analysis showed that elevated NIHSS score, IL-1β, IL-18, TNF-α, and NLRP3 were independent risk factors for poor prognosis in patients with ACI [OR(95%CI)=1.244(1.034-1.497), 1.373(1.067-1.767), 1.047(1.011-1.086), 1.577(1.061-2.343), 1.084(1.022-1.149)], miR-22-3p was the independent protection factor [OR(95%CI)=0.933(0.888-0.980)]. ROC curve analysis showed that the area under the curve of serum miR-22-3p and NLRP3 levels combined to predict the poor prognosis of ACI patients was 0.875, which was greater than that predicted by the two alone(0.786 and 0.759(Z/P=2.405/0.016 and 2.517/0.012). Conclusion The decrease of serum miR-22-3p level and the increase of NLRP3 level in ACI patients are closely related to the increase of inflammatory factor levels and poor prognosis, and the combined serum levels of miR-22-3p and NLRP3 have a high predictive value for poor prognosis in ACI patients.

Objective To explore the expression and clinical significance of serum ubiquitin carboxyl terminal hydrolase-L1(UCH-L1) and tissue non-specific alkaline phosphatase(TNAP) in elderly patients with sepsis associated encephalopathy(SAE). Methods One hundred and seventy-seven elderly patients with sepsis treated in the Department of Geriatrology at the Air Force 986th Hospital of the Air Force Medical University from March 2019 to March 2021 were selected as the research subjects. They were divided into SAE group(n=80) and non-SAE group(n=97) based on whether they were combined with SAE. According to the survival status within 28 days, the SAE group was divided into survival subgroup(n=48) and death subgroup(n=32). Enzyme linked immunosorbent assay was used to detect the expression of serum UCH-L1 and TNAP. The correlation between indicators was analyzed using Pearson correlation analysis. Multivariate logistic regression analysis of factors affecting the prognosis of death in SAE patients. The evaluation value of serum UCH-L1, TNAP, and their combination in predicting the mortality prognosis of SAE patients by analyzing the working characteristic curve of the subjects. Results Serum UCH-L1, TNAP, C-reactive protein, procalcitonin, neuron specific enolase(NSE), SOFA score and APACHE Ⅱ score in SAE group were higher than those in non SAE group(t/P=14.268/<0.001, 18.872/<0.001, 4.607/<0.001, 11.589/<0.001, 7.689/<0.001, 7.572/<0.001, 8.177/<0.001). The serum UCH-L1 and TNAP levels in SAE patients were significantly positively correlated with procalcitonin, C-reactive protein, NSE, SOFA score, and APACHE II score(r/P=0.547/<0.001, 0.661/<0.001, 0.602/<0.001, 0.514/<0.001, 0.498/<0.001; 0.477/<0.001, 0.529/<0.001, 0.632/<0.001, 0.607/<0.001, 0.474/<0.001). The serum procalcitonin, C-reactive protein, NSE, UCH-L1, TNAP, APACHE II score, and SOFA score of SAE patients in the death subgroup were higher than those in the survival subgroup(t/P=7.586/<0.001, 3.311/0.001, 6.735/<0.001, 13.569/<0.001, 11.592/<0.001, 5.205/<0.001, 12.180/<0.001). Elevated serum NSE, UCH-L1, TNAP, APACHE II scores, and SOFA scores are independent risk factors affecting the prognosis of SAE patients [OR(95%CI)=1.868(1.323-2.638), 1.840(1.193-2.838), 1.578(1.122-2.219), 1.659(1.119-2.576), 1.606(1.105-2.336)].The AUC of serum UCH-L1, TNAP, and their combined evaluation of mortality prognosis in SAE patients were 0.848, 0.813, and 0.904, respectively. The combination of the two was superior to their respective individual evaluation values(Z/P=3.864/0.003, 4.270/<0.001). Conclusion The expression of serum UCH-L1 and TNAP is elevated in SAE patients, which is related to the severity of the condition. The combination of serum UCH-L1 and TNAP has high evaluation value for the prognosis of death in elderly SAE patients.