Objective To investigate the expression levels of long-chain non coding RNA(lncRNA) lysine oxidase like 1-antisense RNA1(LOXL1-AS1) and microRNA-3614-5p in the serum of patients with acute myocardial infarction, as well as their predictive value for arrhythmia. Methods One hundred and forty-eight patients with acute myocardial infarction who were hospitalized in the Department of Cardiology at the First Affiliated Hospital of Xi'an Jiaotong University from January 2021 to January 2023 were selected as the study subjects(acute myocardial infarction group). According to whether the patients developed arrhythmia, they were divided into non arrhythmia subgroup(n=96) and arrhythmia subgroup(n=52). In addition, 148 healthy individuals who matched the general information of acute myocardial infarction patients during the same period were selected as the healthy control group. Compare the levels of serum lncRNA LOXL1-AS1 and miR-3614-5p in each group; Multivariate logistic regression analysis of the influencing factors of arrhythmia after acute myocardial infarction; Draw receiver operating characteristic(ROC) curves to analyze the predictive value of serum lncRNA LOXL1-AS1 and miR-3614-5p levels for arrhythmia after acute myocardial infarction. Results Compared with the healthy control group, the level of lncRNA LOXL1-AS1 increased and the level of miR-3614-5p decreased in the acute myocardial infarction group(t/P=16.248/<0.001, 8.397/<0.001); The number of vascular branches and lncRNA LOXL1-AS1 levels in the arrhythmia subgroup were higher than those in the non arrhythmia subgroup, while LVEF and miR-3614-5p levels were lower than those in the non arrhythmia subgroup [χ2(t)/P=14.315/<0.001, 7.312/<0.001, 3.706/<0.001, 7.656/<0.001]; The prediction results from Target Scan Human website show that lncRNA LOXL1-AS1 has a binding site with miR-3614-5p, indicating a possible targeting relationship; The results of multivariate logistic regression analysis showed that high lncRNA LOXL1-AS1 and multiple diseased vessel branches were risk factors for arrhythmia after acute myocardial infarction, while high miR-3614-5p and LVEF were protective factors [OR(95%CI)=3.542(1.589-7.896), 1.527(1.081-2.156), 0.721(0.601-0.865), 0.789(0.664-0.938)]; The AUC of lncRNA LOXL1-AS1, miR-3614-5p, and their combination in predicting arrhythmia after acute myocardial infarction were 0.820, 0.890, and 0.932, respectively. The combination of the two was superior to their individual predictors(Z/P=3.470/0.001, 2.293/0.022). Conclusion The serum lncRNA LOXL1-AS1 levels significantly increased and miR-3614-5p levels significantly decreased in patients with arrhythmia after acute myocardial infarction. The combination of the two has good predictive value for arrhythmia after acute myocardial infarction.

Objective To analyze the relationship between serum miR-126, miR-222, cardiac function, and major adverse cardiovascular events(MACE) in patients with chronic heart failure(CHF) and atrial fibrillation(AF). Methods 120 patients with CHF combined with AF admitted to the Cardiology Department of Lianyungang Hospital Affiliated to Xuzhou Medical University from January 2020 to October 2022 were selected as the AF group. According to the New York Heart Association(NYHA) cardiac function classification, they were divided into Grade Ⅰ 22 cases, Grade Ⅱ 29 cases, Grade Ⅲ 26 cases, and Grade Ⅳ 43 cases. Follow up for 3 months and divide into MACE subgroup and non-MACE subgroup based on the occurrence of MACE. 100 CHF patients without concomitant AF admitted during the same period were selected as the non-AF group. Another 80 healthy volunteers who underwent physical examinations in hospitals during the same period were selected as the healthy control group. Detect and compare the serum levels of miR-126 and miR-222 in three groups of CHF patients with AF and different NYHA heart function grades. Analyze the correlation between serum miR-126, miR-222 levels and NYHA cardiac function grading in patients with CHF combined with AF. Multivariate logistic regression analysis on the influencing factors of MACE in patients with CHF and AF. The predictive value of receiver operating characteristic(ROC) curve analysis of serum miR-126 and miR-222 levels for MACE in patients with CHF and AF. Results The levels of serum miR-126 and miR-222 in the AF group

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Objective To analyze the relationship between serum stress-induced protein 2(Sestrin2) and soluble signaling hormone 4d(sSema4D) levels and coronary slow flow phenomenon(CSFP), and its predictive value for CSFP. Methods Forty patients who underwent coronary angiography at the Cardiology Department of Chifeng College Affiliated Hospital from February 2020 to November 2022 without obvious lesions but with CSFP were selected as the CSFP group. Forty patients without coronary artery stenosis and CSFP were selected as the non CSFP group. All patients underwent myocardial infarction thrombolysis test(TIMI) to detect serum levels of Sestrin2 and sSema4D. Pearson analyzed the relationship between serum Sestrin2, sSema4D, and TIMI. Multivariate logistic regression analysis was used to identify risk factors affecting the occurrence of CSFP. Receiver operating characteristic curve(ROC) analysis was used to assess the value of serum Sestrin2 and sSema4D in predicting CSFP. Results The serum levels of Sestrin2 and sSema4D in the CSFP group were higher than those in the non CSFP group(t/P=21.058/<0.001, 22.299/<0.001). The average TIMI frame rate in the CSFP group was higher than that in the non CSFP group(t/P=15.140/<0.001), and the proportion of TIMI blood flow grade ≤ 2 was higher than that in the non CSFP group(Z/P=80.000/<0.001). The serum levels of Sestrin2 and sSema4D in the CSFP group were positively correlated with the average TIMI frame rate(r/P=0.442/<0.001, 0.316/<0.001). Male, vascular disease, high Sestrin2, and high sSema4D are risk factors for CSFP [OR(95%CI)=2.784(1.386-5.594), 2.038(1.086-3.823), 1.702(1.113-2.605), 1.531(1.070-2.192)]. The AUC of serum Sestrin2 and sSema4D alone and in combination for predicting CSFP were 0.707, 0.753, and 0.873, respectively. The combination of the two was superior to their respective individual predictive values(Z/P=3.047/<0.001, 2.402/0.012). Conclusion The serum levels of Sestrin2 and sSema4D in CSFP patients are both elevated, and are associated with an increase in TIMI grading and the occurrence of CSFP.

Objective To explore the influencing factors of coronary artery positive remodeling in patients with coronary heart disease(CHD) and type 2 diabetes, and to construct related clinical prediction models to identify high-risk patients early and guide clinical treatment. Methods One hundred and four patients with coronary heart disease and type 2 diabetes diagnosed in the Cardiovascular Center of Beijing Tongren Hospital affiliated to Capital Medical University from January 2016 to June 2023 were selected. The target lesions were measured by intracoronary ultrasound(IVUS), and the reconstruction index(RI) was calculated. According to RI, patients were divided into positive reconstruction group and non positive reconstruction group. Clinical data of patients were collected, and the data of the two groups were statistically analyzed and a clinical prediction model was constructed. Results Low blood calcium [OR(95%CI)=1.544(1.263-1.927), P<0.001], concurrent acute coronary syndrome(ACS) [OR(95%CI)=1.198(1.024-1.401), P=0.024], high glycated hemoglobin(HbA1c) [OR(95%CI)=1.498(1.104-2.032), P=0.010] and high and low density lipoprotein cholesterol(LDL-C) [OR(95%CI)=1.275(1.139-1.428), P<0.001] are independent risk factors for positive remodeling of coronary artery in this population. Based on the above risk factors, a nomograph model of positive remodeling of coronary artery in patients with coronary heart disease and type 2 diabetes was constructed. The predicted incidence rate was basically consistent with the actual incidence rate. The area under the internal validation curve(AUC) of the model was 0.937, And it has good clinical applicability. Conclusion The nomogram model based on the risk factors of patients with coronary heart disease and type 2 diabetes has a good predictive effect on the occurrence of positive coronary remodeling.

To analyze the relationship between serum soluble growth stimulating factor 2(sST2) and homocysteine(Hcy) expression levels and cardiovascular damage in children with Kawasaki disease(KD). Methods Retrospective analysis of clinical data of 104 KD patients from January 2020 to December 2022 at the Affiliated Women and Children's Hospital/Chengdu Women and Children's Center Hospital of University of Electronic Science and Technology of Electronic Science and Technology of China. Based on their cardiovascular damage, they were divided into a cardiovascular damaged group(damaged group) of 37 cases and a cardiovascular undamaged group(undamaged group) of 67 cases. Compare the differences in serum sST2 and Hcy levels between two groups of KD children, and use Spearman rank correlation analysis to evaluate the correlation between the severity of cardiovascular damage and serum sST2 and Hcy levels; Use receiver operating curve(ROC curve) to evaluate the diagnostic value of serum sST2 and Hcy levels for cardiovascular damage in children with KD. Results The age of children in the damaged group was lower than that in the undamaged group(t/P=8.401/<0.001), and the proportion of patients who received the first intravenous injection of immunoglobulin(IVIG) for ≥ 10 days was higher than that in the undamaged group(χ2/P=7.355/0.007). The serum levels of sST2 and Hcy in the damaged group were higher than those in the undamaged group(t/P=5.047/<0.001, 5.503/<0.001), and increased with the severity of coronary artery disease in the damaged group(F/P=50.642/<0.001, 55.362/<0.001); The Spearman rank correlation analysis results showed a significant positive correlation between serum sST2 and Hcy levels and the severity of cardiovascular damage in KD children(r/P=0.856/<0.001, 0.870/<0.001); The combined prediction of serum sST2, Hcy, and two items for cardiovascular damage AUC was 0.895, 0.814, and 0.911, respectively. The combined AUC of the two items was greater than its predicted AUC alone(Z=2.453, 2.331, P=0.014, 0.020). Conclusion Serum sST2 and Hcy play important roles in the diagnosis and treatment of cardiovascular damage in children with KD, and have high clinical application value.