Objective To analyze the expression of Frizzled homolog protein 7(FZD7) and galactin 8(GAL-8) in intrahepatic cholangiocarcinoma(ICC), and their relationship with clinical pathological features and prognosis. Methods Eighty-four ICC patients who visited Department of Oncology Yulin First Hospital from April 2017 to April 2020 were selected. Immunohistochemical methods were used to detect the expression of FZD7 and GAL-8 proteins in tissues. The relationship between FZD7 and GAL-8 were conducted by Spearman correlation analysis. Kaplan-Meier method was used to analyze the differences in survival prognosis among ICC patients with different FZD7 and GAL-8 expressions. The prognostic factors for ICC were analyzed by Cox regression analysis. Results The positive rates of FZD7 and GAL-8 in ICC cancer tissue were 73.81%(62/84) and 71.43%(60/84), respectively, which were higher than those in adjacent cancer tissue by 4.76%(4/84) and 7.14%(6/84)(χ2=83.950, 72.770, all P<0.001). FZD7 was positively correlated with GAL-8 expression in ICC(rs=0.745, P<0.001). The positive rates of FZD7 and GAL-8 were higher in ICC cancer tissues with low differentiation degree, lymph node metastasis and TNM stage Ⅲ(FZD7:χ2/P=8.221/<0.001,6.097/0.014,13.014/<0.001;GAL-8:χ2/P=7.207/0.007,5.555/0.018,11.760/0.001). The 3-year overall survival rate of the FZD7 positive group was 32.26%(20/62), which was lower than the negative group's 68.19%(15/22)(Log rank χ2=8.723, P=0.003). The 3-year overall survival rate of the GAL-8 positive group was 28.33%(17/60), which was lower than the GAL-8 negative group's 75.30%(18/24)(Log rank χ2=24.310, P<0.001). TNM stage Ⅲ, low differentiation degree, lymph node metastasis, FZD7 positivity, and GAL-8 positivity were independent risk factors affecting the prognosis of ICC patients [HR(95%CI)=1.614(1.215-2.145),1.516(1.219-1.884),1.916(1.315-2.791),1.826(1.222-2.729),1.737(1.237-2.438)]. Conclusion FZD7 and GAL-8 are upregulated in ICC, both of which are involved in the progression of ICC tumors and serve as tumor markers for evaluating the prognosis of ICC patients.

Objective To investigate the predictive value of serum levels of chitinase 1(CHIT1) and angiotensin Ⅱ receptor-like 1 endogenous ligand 13(Apelin-13) for delayed healing in traumatic fracture patients. Methods A total of 102 elderly patients with traumatic fracture admitted to the Department of Orthopedics of the Fifth Affiliated Hospital of Xinjiang Medical University from April 2020 to April 2023 were selected. They were separated into a delayed healing group(34 cases) and a normal healing group(68 cases) based on their healing status. Enzyme linked immunosorbent assay(ELISA) was applied to detect the levels of CHIT1 and Apelin-13 in serum; Multivariate Logistic regression was used to analyze the factors affecting delayed healing in patients with traumatic fractures. receiver operating characteristic(ROC) curve was applied to analyze the predictive value of CHIT1 and Apelin-13 in serum for delayed healing in patients with traumatic fractures. Results Compared with the normal healing group, the delayed healing group showed an obvious increase in serum CHIT1 level and an obvious decrease in Apelin-13 level(t/P=10.905/<0.001,6.497/<0.001). Multivariate Logistic regression analysis showed that the high level of CHIT1[OR(95%CI)=2.263(1.351-3.789)]and the high level of Apelin-13[OR(95%CI)=0.685(0.552-0.850)]were the influencing factors for delayed healing in patients with traumatic fractures. ROC curve analysis results showed that the area under the curve(AUC) of the combination of the two in predicting delayed healing in traumatic fracture patients was higher than AUC predicted by CHIT1 and Apelin-13 alone(Z=7.788,P<0.001;Z=3.161,P=0.002). Conclusion CHIT1 is highly expressed in the serum of elderly patients with delayed healing of traumatic fractures, while Apelin-13 is lowly expressed. Both have certain predictive value for delayed healing of traumatic fracture patients.

Objective To investigate the efficacy of high-intensity focused ultrasound(HIFU) ablation combined with drospirenone ethinylestradiol tablets in treating adenomyosis(AD) patients and its impacts on angiogenic factors and uterine artery hemodynamics. Methods A total of 104 AD patients diagnosed and treated in the Obstetrics and Gynecology Department of the Fifth People's Hospital of Chengdu from February 2021 to February 2023 were selected as the study objects. They were divided into single group(n=52) and combined group(n=52) by random number table method. The single group was treated with HIFU ablation, while the combination group was treated with drospirenone ethinylestradiol tablets on the basis of the single group. Clinical efficacy, angiogenic factors[serum angiopoietin-2(Ang-2), vascular endothelial growth factor(VEGF), adiponectin(ADPN)], uterine artery hemodynamics[peak blood flow velocity(PSV), end diastolic blood flow velocity(EDV), pulsatile index(PI), resistance index(RI)], ovarian related indicators[estrogen(E2), follicle stimulating hormone(FSH), anti-Mullerian hormone(AMH), luteinizing hormone(LH)]levels, uterine volume, lesion volume and diameter, clinical symptoms(menstrual cycle changes, menstrual prolongation, menstrual blood clotting, increased menstrual flow, daytime urinary frequency, fatigue, pelvic pain, nighttime urinary frequency) scores, and adverse reactions were compared. Results The total effective rate of single group was obviously lower than that of combination group(90.38% vs. 75.00%, χ2/P=4.300/0.038). After treatment, the Ang-2, VEGF, E2, AMH, FSH, LH, PSV, EDV, RI, uterine volume, lesion volume, lesion diameter level, and clinical symptom score of the two groups were obviously lower than before treatment, and the combination group were obviously lower than the single group(t/P=3.340/0.001, 5.125/<0.001, 4.427/<0.001, 9.381/<0.001, 2.313/0.023, 9.795/<0.001, 3.149/0.002, 3.584/0.001, 2.139/0.035, 4.151/<0.001, 15.493/<0.001, 8.814/<0.001, 6.605/<0.001, 4.583/<0.001, 9.112/<0.001, 7.522/<0.001, 5.427/<0.001, 3.425/0.001, 4.084/<0.001, 8.209/<0.001). 2="" after="" the="" adpn="" and="" pi="" of="" groups="" were="" higher="" than="" those="" before="" combined="" group="" was="" significantly="" single="" p="" .="" there="" no="" significant="" difference="" in="" total="" incidence="" adverse="" reactions="" between="">0.05). Conclusion The clinical symptoms of AD patients are obviously improved after HIFU ablation combined with drospirenone ethinylestradiol treatment, and their levels of angiogenic factors and hemodynamics are obviously reduced, indicating high safety.

Objective To investigate the effect and mechanism of Nobiletin(Nob) on necrotic apoptosis in chronic heart failure(CHF) rats by regulating the receptor interacting protein kinase 1/receptor interacting protein kinase 3/mixed lineage kinase like domain protein(RIP1/RIP3/MLKL) signaling pathway. Methods From September 2022 to March 2023, experiment was conducted in Central Laboratory of Southern Medical University. A CHF rat model was established, and 75 successfully modeled rats were randomly divided into model group(CHF group), low-dose Nob group(Nob-L group, 7.5 mg·kg-1·d-1 Nob), medium dose Nob group(Nob-M group, 15 mg·kg-1·d-1 Nob), high-dose Nob group(Nob-H group, 30 mg·kg-1·d-1 Nob), and pathway inhibitor Nec-1 group(Nec-1 group, 2.45 mg·kg-1·d-1 Nec-1) according to the random number table method, with 15 rats in each group. Another 15 rats were selected as the sham operation group(Sham group), with only the thoracic cavity opened and no ligation performed. Echocardiography was applied to detect and detect left ventricular function. Enzyme linked immunosorbent assay(ELISA) was applied to detect the levels of serum tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), malondialdehyde(MDA), superoxide dismutase(SOD), and total antioxidant capacity(T-AOC). Hematoxylin eosin(HE) and Masson staining were applied to observe the pathological changes and fibrosis of myocardial tissue. The terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling(TUNEL) was applied to observe the apoptosis of myocardial tissue. Western blot was applied to detect RIP1, RIP3, MLKL phosphorylation levels, and Caspase-8 protein expression. Results Compared with the Sham group, some cells in the myocardial tissue of the CHF group were damaged, necrotic, structurally disordered, with myocardial cell swelling, a large number of inflammatory cell infiltration, and collagen deposition and fibrosis in myocardial tissue increased, Compared with the CHF group, the myocardial fiber arrangement of the Nob-L group, Nob-M group, Nob-H group, and Nec-1 group gradually became regular, with myocardial cell swelling, necrosis, and the inflammatory cell infiltration decreased, collagen deposition and fibrosis decreased. Compared with the Sham group, the levels of left ventricular end diastolic diameter(LVEDD), left ventricular end systolic diameter(LVESD), IL-1β, TNF-α and MDA, cell apoptosis rate, and the expression of p-RIP1/RIP1, p-RIP3/RIP3, and p-MLKL/MLKL proteins of the CHF group, Nob-L group, Nob-M group, Nob-H group and Nec-1 group were obviously increased(P<0.05), the levels of left ventricular ejection fraction(LVEF), left ventricular axis shortening fraction(FS), SOD, T-AOC, and the expression of Caspase-8 protein of the CHF group, Nob-L group, Nob-M group, Nob-H group and Nec-1 group were obviously reduced(F/P=102.557/<0.001, 117.684/<0.001, 364.401/<0.001, 268.087/<0.001, 124.566/<0.001, 229.003/<0.001, 193.585/<0.001, 182.164/<0.001, 142.657/<0.001, 140.900/<0.001, 169.680/<0.001, 103.485/<0.001, 108.277/<0.001, 200.435/<0.001), and the intervention effect of different dosage groups of Nob showed a dose-dependent relationship(P<0.05). Conclusion Nob may alleviate inflammation and oxidative stress by inhibiting the activation of the RIP1/RIP3/MLKL signaling pathway, inhibit necrotic apoptosis in CHF rats, and exert a protective effect on CHF rats.

Report clinical data of a patient with yellow nail syndrome and conduct literature review.