Objective To explore the expression levels of miR-155 and similar protein 1(BMAL1) of aromatic hydrocarbon receptor nuclear transporters in brain and muscle tissue in male patients with chronic obstructive pulmonary disease(COPD) complicated with insomnia and its correlation with insomnia. Methods Sixty male COPD patients diagnosed and treated in the Neurology Department of the Second Affiliated Hospital of Xinjiang Medical University from June 2021 to April 2023 were selected as the observation group. In addition, 60 male COPD patients with acute episodes of non-insomnia during the same period were selected as the control group. Collect serum extracellular vesicles and alveolar lavage fluid supernatant samples from two groups of patients, and detect and compare the differences in miR-155 and BMAL1 mRNA transcription levels in the samples. Screening the risk factors for insomnia in COPD patients through Spearman correlation analysis and binary logistic regression analysis. Evaluate the predictive value of various risk factors on the occurrence of insomnia through the receiver operating curve(ROC) and area under the curve(AUC). Results The expression levels of miR-155 and BMAL1 mRNA in serum extracellular vesicles and alveolar lavage fluid supernatant of the observation group were significantly higher than those in the control group(t/P=3.457/<0.001, 4.147/<0.001, 4.471/<0.001, 4.336/<0.001). Correlation analysis and binary logistic regression analysis showed that elevated levels of miR-155 and BMAL1 mRNA expression in serum extracellular vesicles and alveolar lavage fluid supernatant were risk factors for insomnia in COPD patients [OR(95%CI)=1.578(1.061-2.123), 1.955(1.208-3.164), 2.476(1.430-4.287), 2.574(1.357-4.885)],The AUC of miR-155 and BMAL1 mRNA in serum extracellular vesicles and alveolar lavage fluid supernatant for predicting the occurrence of insomnia in COPD patients was 0.679,0.706,0.719,0.733 and 0.839,the combination of the four factors has the highest value(Z=2.932,2.771,2.693,2.553,P<0.01).Conclusion The elevated levels of miR-155 and BMAL1 in the serum exosomes and alveolar lavage fluid of COPD patients are significantly correlated with the occurrence of insomnia, suggesting that miR-155 and BMAL1 may be involved in the occurrence and development of COPD related insomnia, providing potential targets for further exploration of the neuropathological mechanisms of insomnia in COPD patients in the future.

Objective To analyze the expression of abundant transcript 1(LncNEAT1) and long chain non coding RNA SRY box transcription factor 2 overlapping transcript 2(LncSOX2OT) in serum extracellular vesicles of elderly sepsis associated encephalopathy(SAE) patients and their clinical prognostic significance.Methods Ninety-six elderly SAE patients who visited the Intensive Care Department of the Fifth People's Hospital Affiliated to Chengdu University of Traditional Chinese Medicine from February 2020 to February 2022 were selected as the SAE group. They were further divided into survival subgroups(n=50) and death subgroups(n=46) based on their survival status within 28 days. 60 sepsis patients without SAE who were diagnosed and treated during the same period were selected as the non-SAE group. Real time fluorescence quantitative PCR(qRT-PCR) was used to detect the expression levels of LncNEAT1 and LncSOX2OT in serum extracellular vesicles. Multivariate logistic regression analysis of prognostic factors in elderly SAE patients. The diagnostic value of receiver operating characteristic curve(ROC) analysis of serum exosomes LncNEAT1 and LncSOX2OT in the prognosis of elderly SAE patients.Results The relative expression of serum extracellular vesicles LncNEAT1 and LncSOX2OT in the SAE group was higher than that in the non SAE group(t/P=16.726/<0.001, 21.803/<0.001). C-reactive protein, procalcitonin, neuron specific enolase, APACHE Ⅱ score, SOFA score, LncNEAT1, LncSOX2OT in the death subgroup of SAE patients were higher than those in the survival subgroup(t/P=14.197/<0.001, 4.535/<0.001, 19.253/<0.001, 8.442/<0.001, 5.670/<0.001, 9.861/<0.001, 6.931/<0.001). The serum exosomes LncNEAT1 and LncSOX2OT in SAE patients were positively correlated with APACHE II score and SOFA score(r/P=0.812/<0.001, 0.761/<0.001, 0.833/<0.001, 0.598/<0.001). High levels of procalcitonin, C-reactive protein, neuron specific enolase, APACHE Ⅱ, SOFA, LncNEAT1, and LncSOX2OT are independent risk factors affecting the 28 day survival prognosis of SAE patients [OR(95%CI)=1.459(1.195-1.782), 1.464(1.164-1.841), 1.334(1.086-1.639), 1.644(1.223-2.210), 1.779(1.295-2.444), 1.347(1.050-1.728), 1.578(1.122-2.219)]. The combined prediction of serum extracellular vesicles LncNEAT1 and LncSOX2OT for 28 day survival prognosis in SAE patients had an AUC of 0.914, which was higher than the single indicator of 0.846 and 0.834(Z/P=3.864/<0.001, 3.915/<0.001).Conclusion The increased expression of serum extracellular vesicles LncNEAT1 and LncSOX2OT in elderly SAE patients is an independent risk factor affecting the prognosis of elderly SAE patients, and has high evaluation value for the survival and prognosis of elderly SAE patients.

Objective To explore the expression and clinical significance of serum albumin(Asposin), microRNA-206(miR-206), and ischemic modified albumin(IMA) in patients with polycystic ovary syndrome(PCOS). Methods One hundred and twenty PCOS patients admitted to Qingdao Municipal Hospital from June 2019 to June 2022 were selected as the case group. They were divided into overweight/obese subgroups and normal subgroups based on body mass index(BMI), and insulin resistance(IR) subgroups and non IR subgroups based on insulin resistance index(HOMA-IR). Additionally, 60 healthy women of childbearing age during the same period were selected as the healthy control group. Detect and compare the expression levels of Asposin, miR-206, and IMA in serum of each group, and their correlation with various indicators. Multivariate logistic regression analysis was used to analyze the risk factors for IR in PCOS patients. The receiver operating characteristic curve(ROC) was used to analyze the predictive value of each indicator on the occurrence of IR in PCOS. Results The BMI, HOMA-IR, total cholesterol, triacylglycerol, Luteinizing hormone, follicle stimulating hormone, testosterone, hs CRP, Asprosin, IMA in the case group were higher than those in the healthy control group, and miR-206 was lower than that in the healthy control group(t=2.029, 9.850, 5.568, 12.664, 7.221, 8.900, 6.973, 9.876, 9.876, 16.038, 35.177, all P<0.05). The Asprosin and IMA levels in PCOS patients in the overweight/obesity subgroup were higher than those in the normal body mass subgroup(t=4.399, 3.054, P<0.01). The Asposin and IMA levels in the IR subgroup of PCOS patients were higher than those in the non-IR subgroup, while miR-206 levels were lower than those in the non-IR subgroup(t=5.467, 3.514, 10.158, P<0.01). Asposin was positively correlated with BMI, HOMA-IR, total cholesterol, and triglycerides(r=0.425, 0.524, 0.405, 0.423, P<0.01), miR-206 was negatively correlated with HOMA-IR, Asposin, and IMA(r=-0.332,-0.415,-0.433, P<0.001), and IMA was positively correlated with HOMA-IR, Asposin(r=0.624, 0.394, P<0.05). High Asposin, low miR-206, and high IMA are risk factors for the occurrence of IR in PCOS [OR(95% CI)=2.385(1.191-4.722), 3.367(1.580-7.175), 2.153(1.053-4.404)]. The area under the curve(AUC) for predicting IR in PCOS were 0.761, 0.894, 0.667, and 0.918, respectively, and jointly predicted AUC was higher than Asposin and IMA(Z=3.831, 4.663, P<0.01).Conclusion Asposin and IMA are abnormally high expressed in the serum of PCOS patients, while miR-206 is abnormally low expressed. Their expression is related to the degree of IR and may be involved in the occurrence and development of IR in PCOS patients, which has certain value in predicting the occurrence of IR in PCOS patients.

Objective To investigate the levels and clinical significance of serum positive pentameric protein 3(PTX3) and multi ligand proteoglycan 1(SDC-1) in children with idiopathic thrombocytopenic purpura(ITP). Methods Ninety-five children with ITP admitted to the Department of Pediatrics at Cangzhou Central Hospital from October 2021 to October 2022 were selected as the study group; 100 healthy children who underwent physical examination at the same hospital were selected as the healthy control group. Enzyme linked immunosorbent assay(ELISA) was used to detect the levels of serum PTX3 and SDC-1 in two groups. Pearson correlation analysis was used to investigate the correlation between serum PTX3 and SDC-1 levels. Logistic regression model was used to analyze the relevant influencing factors of prognosis in children with ITP; The diagnostic value and prognostic predictive value of serum PTX3 and SDC-1 levels in ITP were analyzed using the receiver operating characteristic(ROC) curve analysis. Results Compared with the healthy control group, the study group showed a decrease in platelet count(PLT) levels and a significant increase in serum PTX3 and SDC-1 levels in ITP patients(t/P=76.639/<0.001, 8.868/<0.001, 8.483/<0.001). Compared with the subgroup with good prognosis, the serum PTX3 and SDC-1 levels in the subgroup with poor prognosis ITP were significantly increased(t/P=8.313/<0.001, 7.851/<0.001). Pearson correlation analysis showed a positive correlation between serum PTX3 and SDC-1 levels in children with ITP(r/P=0.700/<0.001). The AUC of serum PTX3, SDC-1, and their combination in diagnosing ITP were 0.801, 0.849, and 0.861, respectively. The combination of the two was superior to serum PTX3 alone in diagnosing ITP(Z=2.708, P=0.007). The AUC of serum PTX3, SDC-1, and their combination in predicting the prognosis of children with ITP were 0.790, 0.907, and 0.918, respectively. The combination of the two was better than serum PTX3 alone in predicting prognosis(Z=2.245, P=0.025). Logistic regression analysis showed that high levels of serum PTX3 and SDC-1 were risk factors for poor prognosis in children with ITP [OR(95%CI)=5.463(3.193-9.347), 13.486(10.473-17.366)].Conclusion The elevated levels of serum PTX3 and SDC-1 in children with ITP have important clinical significance for the diagnosis and prognosis prediction of ITP.

Objective To observe the effect of Leonurine regulated protein kinase B(Akt)/bimicrosomal homologous gene 2(MDM2)/p53 signaling pathway on the malignant biological behavior of glioma cells. Methods The experiment was conducted in the laboratory of Taihe Hospital in Shiyan City, Hubei Province from March to September 2022. The CCK-8 method was used to determine the survival rate of mouse glioma cells GL261 treated with Leonurine at different concentrations(0, 0.4, 0.8, 1.2, 1.6, 2.0 mmol/L) and screen for their optimal concentration of action. GL261 cells were cultured in vitro and randomly divided into control group, Leonurine group, and Leonurine +SC79(Akt activator) group, with 1.6 mmol/L Leonurine and 5 μmol/L SC79 after grouping, protein immunoblotting was used to detect the expression of Akt/MDM2/p53 pathway related proteins in each group of cells; CCK-8 method, flow cytometry, cell scratch assay, and Transwell assay were used to detect the proliferation, apoptosis, migration, and invasion of cells in each group. 30 intracranial glioma mouse models were constructed and randomly divided into a control group, a low-dose group of Leonurine, a medium dose group of Leonurine, a high-dose group of Leonurine, and a high-dose+SC79 group of Leonurine. After grouping, the apoptosis of intracranial glioma cells in each group of mice was detected using TUNEL staining; Immunoblotting was used to detect the expression of Akt/MDM2/p53 pathway related proteins in glioma tissues of each group. Results(1) Cell experiment: Compared with the control group, the Leonurine group showed an increase in cell apoptosis rate and p53 protein expression(P<0.05), while survival rate, migration rate, invasion number, p-Akt/Akt, and p-MDM2/MDM2 decreased(P<0.05). Compared with the Leonurine group, the Leonurine +SC79 group showed a decrease in cell apoptosis rate and p53 protein expression(P<0.05), while survival rate, migration rate, invasion number, p-Akt/Akt, and p-MDM2/MDM2 increased(P<0.05).(2) Animal experiment: Compared with the control group, the Leonurine group showed an increase in cell apoptosis rate and p53 protein expression in glioma tissue(t/P=20.076/<0.001, 7.486/<0.001), while p-Akt/Akt and p-MDM2/MDM2 decreased(t/P=11.769/<0.001, 7.579/<0.001); Compared with the Leonurine group, the Leonurine +SC79 group showed a decrease in cell apoptosis rate and p53 protein expression in glioma tissue(t/P=18.328/<0.001, 7.359/<0.001), while p-Akt/Akt and p-MDM2/MDM2 increased(t/P=9.640/<0.001, 5.529/<0.001). Conclusion Leonurine can inhibit the proliferation, migration, invasion, and growth of glioma cells in mice by inhibiting Akt/MDM2/p53 signaling, and induce apoptosis, ultimately inhibiting their malignant progression.