Objective To observe the effect of synchronous push radiotherapy on bone metastasis of breast cancer. Methods From December 2019 to March 2022, 92 patients with bone metastases from breast cancer were treated in the Department of Radiotherapy, Friendship Hospital, Ili Kazak Autonomous Prefecture. They were randomly divided into an observation group and a control group, with 46 cases in each group. The control group received routine radiotherapy, while the observation group received synchronous push dose radiotherapy. Calculate the 5-week treatment effect of radiotherapy, as well as the actual radiation dose to the tumor target area(GTV), clinical target area(CTV), and planned target area(PTV). Record the volume percentage of the 10 Gy and 20 Gy cardiac and pulmonary radiation doses; Compare the degree of bone pain, serum prostate specific antigen(PSA), and nerve growth factor(NGF) levels before and after radiotherapy; Count the occurrence of adverse reactions and investigate the repair of osteolytic lesions after 3 months. Results The disease control rate in the observation group was higher than that in the control group(93.48% vs. 78.26%, χ2/P=4.390/0.036). The GTV dose in the observation group was higher than that in the control group, while the PTV dose was lower than that in the control group(t/P=4.932/<0.001, 4.494/<0.001). The volume of the heart and lungs exposed to 20 Gy of radiation in the observation group was significantly smaller than that in the control group(t/P=2.975/0.004, 4.969/<0.001). The degree of bone pain in the observation group after 3 weeks of radiotherapy was milder than that in the control group(Z/P=2.285/0.022). The levels of serum PSA and NGF in the two groups after 3 weeks and 5 weeks of radiotherapy were lower than before radiotherapy, and the levels after 5 weeks of radiotherapy were lower than those after 3 weeks of radiotherapy. The levels in the observation group were lower than those in the control group(F/P=13.746/<0.001, 11.942/0.002); The overall incidence of radiation damage in the observation group is lower than that in the control group(χ2/P=4.483/0.034). The repair rate of osteolytic lesions in the observation group was higher than that in the control group(χ2/P=4.089/0.043).Conclusion Synchronous dose radiotherapy for bone metastasis of breast cancer has a good effect, which can improve the local anti-tumor effect, reduce the radiation dose endangering organs, accelerate the relief of bone pain symptoms, promote bone calcification, and has high safety.

Objective To analyze the value of Topoisomerase II α(TOP2A) combined with miR-21 detection in evaluating the condition and prognosis of cervical cancer. Methods Eighty cervical cancer patients(CVC group) and 45 cervical benign disease patients(CON group) who were diagnosed and treated in the Obstetrics and Gynecology Department of Wuhan Third Hospital and Wuhan University People's Hospital from January 2015 to December 2016 were selected as the research subjects. Real time fluorescence quantitative PCR(qRT-PCR) was used to detect the expression of TOP2A and miR-21 in cervical tissues of the CVC group and CON group, and to compare the differences in tumor tissue TOP2A and miR-21 expression in different clinical and pathological factors in the CVC group. Analyze the relationship between TOP2A, miR-21 expression and survival. The value of using ROC curves to analyze TOP2A and miR-21 in predicting 1-year poor prognosis of cervical cancer; Multivariate logistic regression analysis of risk factors for cervical cancer death. Results The expression of TOP2A and miR-21 in tumor tissues of patients in the CVC group was significantly higher than that in adjacent cancer tissues and the CON group(F/P=83.181/<0.001, 106.861/<0.001). The expression of TOP2A and miR-21 in patients with low differentiation, histological grade G3, depth of muscle infiltration ≥ 1/2, lymph node metastasis, and stage III+IV was significantly higher than that in patients with medium to high differentiation, histological grade G1+G2, and depth of muscle infiltration<1/2 Patients without lymph node metastasis and stage I+II(TOP2A: t/P=15.341/<0.001, 9.687/<0.001, 18.459/<0.001, 9.812/<0.001, 21.722/<0.001; miR-21: t/P=9.052/<0.001, 10.441/<0.001, 17.391/<0.001, 8.457/<0.001, 18.236/<0.001). The median survival of patients with cervical cancer TOP2A ≥ 1.82 and miR-21 ≥ 1.36 in the CVC group was lower than that of patients with TOP2A<1.82 or miR-21<1.36(Log rank=8.645, P=0.003). The area under the curve(AUC) of TOP2A, miR-21, and their combination for predicting 1-year poor prognosis of cervical cancer were 0.815, 0.831, and 0.927, respectively. The combination of the two had the highest AUC(Z/P=2.542/0.018, 2.218/0.021). Low differentiation, histological grade G3, depth of muscle infiltration ≥ 1/2, lymph node metastasis, FIGO stage III+IV, TOP2A expression ≥ 1.82, miR-21 expression ≥ 1.36 are independent risk factors for cervical cancer death [OR(95%CI)=1.978(1.134-5.792), 2.085(1.027-5.092), 2.252(1.131-4.877), 3.425(1.361-5.082), 4.297(1.245-6.138), 2.654(1.189-6.712), 2.512(1.182-5.956)].Conclusion The expression of TOP2A and miR-21 in tumor tissue of cervical cancer patients is significantly increased, which is related to the degree of differentiation, histological grading, depth of muscle invasion, lymph node metastasis, and FIGO staging. It can be used as a biomarker for evaluating the condition and prognosis of cervical cancer. The combination of TOP2A and miR-21 detection can significantly improve the predictive value of poor prognosis in cervical cancer.

Objective To investigate the expression and clinical significance of lysophosphatidylcholine acyltransferase 1(LPCAT1) in cervical cancer. Methods One hundred and sixty-three patients with cervical cancer who underwent gynecological surgery at the First Affiliated Hospital of Hainan Medical College from January 1, 2012 to December 31, 2022 were selected as the observation group, and 77 patients who underwent total hysterectomy for uterine leiomyoma during the same period were selected as the control group. Immunohistochemical method was used to detect the expression level of LPCAT1 protein in cervical cancer tissue and normal cervical tissue, and the differences in clinical pathological characteristics of different cervical cancer patients were compared. Kaplan Meier method was used to analyze the relationship between LPCAT1 protein expression and postoperative prognosis of cervical cancer patients. Cox regression model was used to analyze the influencing factors of postoperative prognosis of cervical cancer patients. Results The high expression rate of LPCAT1 protein in cervical cancer tissue is significantly higher than that in normal cervical tissue(χ2=18.509, P<0.001); high="" expression="" of="" lpcat1="" protein="" in="" cervical="" cancer="" tissues="" with="" figo="" stage="" infiltration="" depth="">1/2 muscle layer, lymph node metastasis, high differentiation in tissues higher than FIGO stage I-II, infiltration depth ≤ 1/2 muscle layer, no lymph node metastasis, and low differentiation(χ2/P=5.501/0.019, 20.463/<0.001, 5.979/0.014, 21.675/<0.001). The overall survival rate of individuals with high expression of LPCAT1 protein is significantly lower than those with low expression(χ2=4.791, P=0.029); FIGO staging stages III-IV, tumor infiltration depth>1/2 muscle layer, lymph node metastasis, medium to high differentiation, and high expression of LPCAT1 protein are independent risk factors for postoperative prognosis of cervical cancer patients [HR(95%CI)=1.564(1.284-2.122), 1.376(1.069-1.972), 2.439(1.300-3.950), 2.690(2.049-3.699), 1.302(1.068-1.590)]. Conclusion The LPCAT1 protein is highly expressed in cervical cancer tissue, and its high expression can indicate poor postoperative prognosis in cervical cancer patients. LPCAT1 protein may have the potential as a biomarker for early diagnosis and prognosis prediction of cervical cancer.

Objective To investigate the relationship between the expression of serum LncRNA XIST and miR-196b, clinical pathological features, and prognosis in patients with acute myeloid leukemia(AML). Methods A total of 88 AML patients admitted to the Hematology Department of Inner Mongolia Medical University Affiliated Hospital from March 2016 to March 2020 were selected as the AML group, while 41 health examination volunteers from the hospital during the same period were selected as the healthy control group. Real time fluorescence quantitative polymerase chain reaction(RT-qPCR) was used to detect the expression of serum LncRNA XIST and miR-196b. Compare the expression differences of serum LncRNA XIST and miR-196b in AML patients with different clinical and pathological characteristics, analyze the correlation between LncRNA XIST and miR-196b, and the relationship between LncRNA XIST and miR-196b and the prognosis of AML patients. Results The expression of serum LncRNA XIST and miR-196b in the AML group was higher than that in the control group(t/P=12.820/<0.001, 14.405/<0.001).The expression of serum LncRNA XIST and miR-196b in AML patients with NCCN grading high-risk, extramedullary infiltration, white blood cell count ≥10×109/L is higher than that in NCCN grading with low to medium risk, no extramedullary infiltration, and white blood cell count<10×109/L AML patients(LncRNA XIST: t/P=8.410/<0.001, 5.830/<0.001, 5.430/<0.001; miR-196b: t/P=7.261/<0.001, 5.658/<0.001, 10.329/<0.001). There is a positive correlation between serum LncRNA XIST and miR-196b expression in AML patients(r/P=0.423/<0.001). The OS rate of AML patients with high LncRNA XIST expression and high miR-196b expression is lower than that of low LncRNA XIST expression and low miR-196b expression(χ2/P=12.290/<0.001, 5.413/0.012). Cox regression analysis showed high risk of NCCN grading, extramedullary infiltration, and white blood cell count ≥ 10×109/L, high LncRNA XIST expression, and high miR-196b expression are risk factors for poor prognosis in AML patients [OR(95%CI)=1.881(1.201-2.947), 1.540(1.113-2.133), 1.484(1.180-1.767), 1.655(1.172-2337), and 1.508(1.171-1.942)].Conclusion The expression of LncRNA XIST and miR-196b in serum of AML patients is increased, which is related to the malignant clinical and pathological characteristics of AML and the occurrence of adverse prognosis.

Objective To explore the value of magnetic resonance imaging(MR) and diffusion weighted magnetic resonance imaging(DWI) in the differential diagnosis of benign and malignant superficial soft tissue tumors. Methods Eighty patients with pathologically confirmed superficial soft tissue tumors admitted to Shijiazhuang People's Hospital from June 2021 to June 2022 were selected as the study subjects, including 50 cases in the benign tumor group and 30 cases in the malignant tumor group. All patients underwent routine MR scanning and DWI examination to compare the MR imaging features of the benign and malignant tumor groups. The maximum enhancement rate(ERmax), maximum slope of increase(Slopemax), and apparent diffusion coefficient(ADC) values were compared between the two groups. The efficacy of subject operating characteristic curve(ROC) analysis of ERmax, Slopemax, and ADC in distinguishing benign and malignant soft tissue tumors was also evaluated. Results There were statistically significant differences in tumor size, tumor boundary, tumor signal, invasiveness, and enhancement mode between the benign tumor group and the malignant tumor group in MR examination(P<0.05). The coincidence rates of MR, DWI, and MR combined with DWI in diagnosing malignant tumors were 66.7%, 73.3%, and 83.3%, respectively. The coincidence rates of MR, DWI, and MR combined with DWI in diagnosing benign tumors were 76.0%, 80.0%, and 88.0%, respectively. MR combined with DWI detection was higher than MR and DWI single detection(Z=3.208, 3.412, P<0.05). The ERmax and Slopemax values in the benign tumor group were lower than those in the malignant tumor group, while the ADC values were higher than those in the malignant tumor group(t/P=6.711/<0.001, 4.220/<0.001, 12.959/<0.001); The AUC values of ERmax, Slopemax, ADC, and their combination in the diagnosis and differentiation of benign and malignant soft tissue tumors were 0.827, 0.744, 0.768, and 0.933, which were higher than those of single detection(Z/P=4.212/<0.001, 3.451/<0.001, 3.425/<0.001). Conclusion MR and DWI parameters are helpful in the differential diagnosis of benign and malignant superficial soft tissue tumors, and the combination of ERmax, Slopemax, and ADC values can improve the accuracy of diagnosis.