Objective To analyze the predictive value of serum thyroglobulin(Tg) and thyroglobulin antibody(TgAb) on the prognosis of patients with differentiated thyroid carcinoma treated with 131I after resection. Methods One hundred and ninety-one patients with differentiated thyroid cancer were treated by thyroid surgery in the First Affiliated Hospital of Xinjiang Medical University from January 2017 to December 2019. They received 131I treatment after surgery and were followed up for 1 year after treatment. According to the follow-up results, the patients were divided into two groups: the group with good prognosis and the group with poor prognosis. The clinical data of the patients in the two groups were compared, and the indexes of thyroid function [triiodothyronine(T3), thyroxine(T4) Free triiodothyronine(FT3), free thyroxine(FT4), thyrotropin(TSH), Tg, TgAb], neutrophil gelatinase-associated lipid carrier protein(NGAL) and imaging indicators(lymph node metastasis, multi-focus metastasis, size of metastasis, number of metastasis). The factors affecting the prognosis of patients were analyzed by using multivariate logistic regression model, The predictive value of the prognostic indicators of patients was analyzed by the ROC. Results During the follow-up, 8 patients were lost and 183 patients were eventually included, including 127 patients with good prognosis and 56 patients with poor prognosis. In the poor prognosis group, the tumor stage Ⅲ to Ⅳ, multiple metastasis, the proportion of lesions>1 cm, and the serum Tg, TgAb, NGAL levels were higher than those in the good prognosis group [χ2(t)/P=12.601/<0.001, 4.165/0.042, 7.741/0.005, 10.657/<0.001, 10.592/<0.001, 8.586/<0.001];There was no significant difference between the two groups in T3, T4, FT3, FT4, TSH, lymph node metastasis and the number of metastatic foci(P>0.05). Multivariate logistic regression analysis showed that high Tg, high TgAb, high NGAL level and multifocal metastasis were independent risk factors for the prognosis of patients [OR(95% CI)=1.114(1.060-1.172), 1.016(1.007-1.025), 1.108(1.042-1.178), 68.700(2.712-1 740.439)], while tumor stage I-II was an independent protective factor for the prognosis of patients [OR(95% CI)=0.026(0.001-0.696)]. The ROC curve showed that the area under the curve(AUC) of serum Tg, TgAb, NGAL and their combination to predict the prognosis of patients were 0.908, 0.852, 0.805 and 0.977, respectively. The combined predictive value of the three indicators was higher than that of single indicators(Z=3.329, 4.013, 4.881, P<0.001). Conclusion The combination of serum Tg and TgAb can effectively predict the prognosis of patients with differentiated thyroid cancer undergoing 131I treatment after operation, which has high diagnostic value and diagnostic efficacy.

cer who underwent surgery in the Department of Urology, West China Hospital, Sichuan University from January 2017 to May 2019 were selected as the study subjects. During the surgery, the bladder cancer tissue and normal bladder tissue adjacent to the cancer were taken. Follow-up for 3 years showed that 89 patients survived as a good prognosis group and 44 patients died as a poor prognosis group. The expression of MYBL2 mRNA and miR-631 in bladder cancer tissues and normal adjacent bladder tissues were detected by fluorescence quantitative PCR. The positive rate of MYBL2 expression in bladder cancer tissues and adjacent normal bladder tissues was detected by immunohistochemistry. Spearman method was used to analyze the correlation between MYBL2 and miR-631 in bladder cancer tissue. Kaplan Meier method was used to analyze the relationship between the expression of MYBL2, miR-631 and the prognosis of bladder cancer; Logistic regression analysis was used to analyze the risk factors of poor prognosis in bladder cancer patients.Results The proportion of patients with T3-4 stage, low differentiation and lymph node metastasis in poor prognosis group was significantly higher than that in good prognosis group(χ2/P=6.590/0.010, 14.208/<0.001, 20.828/<0.001).Compared with normal bladder tissue adjacent to cancer, the expression level of MYBL2 mRNA and the positive expression rate of MYBL2 in bladder cancer tissue were significantly higher [t/P=21.501/<0.001,χ2/P=92.371/<0.001], the expression level of miR-631 was significantly decreased(t/P=7.875/<0.001). MYBL2 and miR-631 have binding sites, and the expression of MYBL2 and miR-631 in bladder cancer tissue is negatively correlated(r/P=-0.424/<0.001). The 3-year survival rate of MYBL2 positive group is lower than that of MYBL2 negative group(χ2/P=5.052/0.025), the 3-year survival rate of miR-631 low expression group is lower than that of miR-631 high expression group(χ2/P=5.934/0.015).Logistic regression analysis showed that TNM stage T3-4, poor differentiation of tumor, lymph node metastasis, positive MYBL2 and low expression of miR-631 in bladder cancer tissue were risk factors for poor prognosis of patients [OR(95% CI)=2.153(1.453-3.191), 2.204(1.274-3.812), 2.458(1.153-5.239), 2.569(1.614-4.088), 2.941(1.798-4.810)].Conclusion MYBL2 and miR-631 are both related to the occurrence of bladder cancer, and detection of their levels is of great significance in evaluating the prognosis of bladder cancer patients.

Objective To investigate the expression level of serum microRNA(miR)-214 and miR-143 in patients with acute myeloid leukemia(AML), and analyze the relationship between them and relapse after AML treatment.Methods Ninety-four AML patients treated in the Department of Hematology, West China Hospital, Sichuan University from January 2019 to April 2021 were selected as the study subjects(AML group). According to whether the AML patients recurred within 1 year after treatment, they were divided into 60 AML remission subgroups and 34 AML recurrence subgroups. In addition, 50 healthy people in the same hospital were selected as the healthy control group. The expression levels of serum miR-214 and miR-143 were detected by real-time fluorescence quantitative PCR; Spearman grade correlation analysis was used to analyze the relationship between the expression of serum miR-214 and miR-143 and the relapse of AML patients after treatment. Multivariate logistic regression analysis was used to analyze the influencing factors of relapse of AML patients. The work characteristic curve(ROC) analysis of subjects was used to analyze the level of serum miR-214 and miR-143 to predict the efficacy of relapse of AML patients after treatment.Results The expression level of serum miR-214 in AML group was higher than that in healthy control group, and the expression level of miR-143 was lower than that in healthy control group(t/P=7.024/<0.001, 7.191/<0.001). The serum miR-214 expression level of AML patients in the relapse subgroup was higher than that in the remission subgroup, and the miR-143 expression level was lower than that in the remission subgroup(t/P=2.940/0.004, 6.596/<0.001). The WBC of AML patients in the relapse subgroup was higher than that in the remission subgroup, and the levels of Hb and PLT were lower than those in the remission subgroup(t/P=3.788/<0.001, 2.093/0.039, 2.974/0.004). Recurrence of AML patients was positively correlated with the expression of serum miR-214(r/P=0.584/<0.001), and negatively correlated with the expression of serum miR-143(r/P=-0.428/<0.001). Multivariate logistic regression analysis showed that high WBC and miR-214 were risk factors for relapse in AML patients [OR(95% CI)=1.647(1.062-2.555), 1.392(1.031-1.879)], and high Hb, PLT and miR-143 were protective factors [OR(95% CI)=0.839(0.805-0.991), 0.714(0.514-0.992), 0.768(0.606-0.974)]. ROC curve analysis showed that the area under the curve of serum miR-214, miR-143 and their combination to predict the recurrence of AML was 0.765, 0.900 and 0.939. The combined prediction of the two was better than the single index prediction(Z/P=3.318/0.002, 2.342/0.019). Conclusion The expression of miR-214 and miR-143 in serum of AML patients were up-regulated and down-regulated, both of which were effective in predicting the recurrence of AML patients.

Objective To analyze the changes of soluble fms like tyrosine kinase-1(sFlt-1), placental growth factor(PLGF) and their ratios in the peripheral blood of pregnant women with early missed abortion and their diagnostic value for early missed abortion. Methods From June 2021 to June 2022, 200 early pregnant women with abnormal medical genetics or physical examination in Haikou Maternal and Child Health Care Institute were selected as the study group, and 200 healthy pregnant women with prenatal examination in the same hospital were selected as the control group. The pregnant women in the study group were continuously monitored until 12 weeks of pregnancy, and were divided into 171 non abortion subgroups and 29 abortion subgroups according to the occurrence of early missed abortion. Compare the changes of maternal serum β-HCG, progesterone, sFlt-1, PLGF, sFlt-1/PLGF. The diagnostic value of serum sFlt-1, PLGF, sFlt-1/PLGF alone or in combination for early missed abortion was analyzed by the subject's work characteristic curve(ROC). Results The levels of study group serum β-HCG, progesterone and PLGF were lower than those in the control group, and the values of sFlt-1 and sFlt-1/PLGF were higher than those in the control group(t/P=31.993/<0.001, 2.906/0.004, 21.297/<0.001, 11.444/<0.001, 22.599/<0.001). 29(14.50%) of the 200 pregnant women in the study group had early missed abortion. Serum sFlt-1 and sFlt-1/PLGF at admission and diagnosis of abortion subgroup were higher than those of non-abortion subgroup at admission and 12 weeks of pregnancy(sFlt-1: t/P=1.980/0.049, 4.487/<0.001; sFlt-1/PLGF: t/P=2.876/0.004, 6.756/<0.001), and PLGF at admission and diagnosis of abortion subgroup was lower than those of non-abortion subgroup at admission and 12 weeks of pregnancy(t/P=2.012/0.046, 7.691/<0.001). In the abortion subgroup, the serum sFlt-1 and sFlt-1/PLGF showed an upward trend at the time of admission, diagnosis and before uterine clearance, and decreased significantly 3 days after uterine clearance(F/P=21.503/<0.001, 16.400/<0.001), while the serum PLGF showed a downward trend(F/P=42.184/<0.001). ROC curve results showed that the AUC of serum sFlt-1, PLGF, sFlt-1/PLGF and their combination in diagnosing early missed abortion were 0.755, 0.858, 0.866 and 0.873, respectively. The combined diagnostic value of the three was higher, but the difference was not statistically significant(Z/P=1.593/0.111, 0.241/0.810, 0.121/0.904). Conclusion Serum sFlt-1, PLGF and their ratios showed dynamic changes before and after the diagnosis of early missed abortion and the curettage, which can be used for the early diagnosis of early missed abortion.

Objective To analyze the expression of serum platelet reactive protein 1(TSP-1) and pentameric protein 3(PTX-3) in patients with hypertensive disorder complicating pregnancy(HDP), and to establish a risk model for predicting adverse pregnancy outcomes. Methods One hundred and forty eight cases of HDP patients diagnosed and treated in the Obstetrics Department of the Second Affiliated Hospital of Hainan Medical College from January 2020 to March 2022 were selected as the observation group, and were divided into 109 cases with good pregnancy outcomes and 39 cases with bad pregnancy outcomes according to pregnancy outcomes. 148 healthy pregnant women were selected as control group. The levels of serum TSP-1, PTX-3 and the incidence of adverse pregnancy outcomes were compared between the two groups. Logistic regression was used to analyze the influencing factors of adverse pregnancy outcome in HDP, and a risk model was constructed. Draw the ROC to analyze the value of serum TSP-1 and PTX-3 in evaluating the adverse pregnancy outcome of HDP patients. Results The levels of serum TSP-1 and PTX-3 in the observation group were higher than those in the control group(t/P=6.146/<0.001, 24.532/<0.001). The levels of serum TSP-1 and PTX-3 in the subgroup with poor pregnancy outcome were higher than those in the subgroup with good pregnancy outcome(t/P=6.224/<0.001, 3.360/0.001). The incidence of premature delivery, fetal distress and total adverse pregnancy outcome in the observation group was higher than that in the control group(χ2/P=11.594/<0.001,7.498/0.006,24.306/<0.001). Logistic regression analysis showed that the increase of serum TSP-1 and PTX-3 was a risk factor for adverse pregnancy outcomes in HDP patients [OR(95%CI)=3.493(1.864-6.544), 3.030(1.607-5.712)]. Establish logistic regression model: logit(P)=-6.384+1.251×TSP-1+1.108×PTX-3, according to the Hosmer-Lemeshow goodness of fit test, the goodness of fit is good. ROC curve analysis showed that AUC value of adverse pregnancy outcome assessed by risk model based on serum TSP-1 and PTX-3 was greater than single prediction(Z/P=2.756/0.005, 2.741/0.004). Conclusion The serum TSP-1 and PTX-3 were highly expressed in HDP patients. The risk model of adverse pregnancy outcome based on the expression of serum TSP-1 and PTX-3 can effectively evaluate the adverse pregnancy outcome of HDP patients.