Objective To analyze the expression and clinical significance of miＲ-34a-5p and miＲ-149-5p in joint
fluid of patients with knee osteoarthritis ( KOA) ． Methods The clinical data of 90 KOA patients from the Orthopedics De-
partment of Wuhan First Hospital from January 2020 to June 2022 were collected． According to the Kellgren-Lawrence ( KL)
standard grading of X-ray film，45 patients were divided into mild KOA group ( grade 1-2 patients) and severe KOA group
( grade 3-4 patients) ． In addition，45 patients with physical examination in the same period were selected as the control
group． The expression of miＲ-34a-5p and miＲ-149-5p in joint fluid of each group was detected by qＲT-PCＲ，and the amount
of expression IL-1β in joint fluid of each group was detected by ELISA． Spearman analyzed the correlation between expression
level of miＲ-34a-5p，miＲ-149-5p and IL-1β in joint fluid，KL grading． The diagnostic value of the expression of miＲ-34a-5p
and miＲ-149-5p in the joint fluid for the severity of KOA was analyzed by the working characteristic curve ( ＲOC) of subjects．
Ｒesults MiＲ-34a-5p，IL-1 in knee joint fluid β Comparison of expression level，severe KOA group ＞ mild KOA group ＞ con-
trol group ( F / P = 19． 21 / ＜ 0． 001，14． 86 / ＜ 0． 001) ． Compared with the expression level of miＲ-149-5p in knee joint fluid，
severe KOA group ＜ mild KOA group ＜ control group ( F / P = 63． 81 / ＜ 0． 001) ． Spearman correlation analysis showed that the
expression level of miＲ-34a-5p and IL-1 β，KL grading showed significant positive correlation ( r / P = 0． 835 / ＜ 0． 001，0．Chin J Diffic and Compl Cas，March 2023，Vol． 22，No． 3

835 / ＜ 0． 001) ，and the expression level of miＲ-149-5p was correlated with IL-1 β，KL grading showed significant negative
correlation ( r / P = － 0． 828 / ＜ 0． 001，－ 0． 943 / ＜ 0． 001) ． The ＲOC curve analysis showed that the area under the curve of
miＲ-34a-5p，miＲ-149-5p and their combined prediction of KOA severity were 0． 902，0． 850 and 0． 976，respectively． The
value of their combined prediction of KOA severity was higher than the single index ( Z / P = 240． 70 /0． 046224． 13 /0． 005) ．
Conclusion The expression of miＲ-34a-5p and miＲ-149-5p in the joint fluid of KOA patients is abnormal，and is significant-
ly related to the clinical grade and laboratory indicators of KOA，which can be used as a potential marker for diagnosis and mo-
nitoring of KOA disease activity．

Health status identification is the basis and key link of implementing health management． Guided by the
eight-character meridian theory of dredging collaterals and preserving health，this paper puts forward the identification of the
healthy state of collaterals，essence，shape and spirit; Differentiation of essence: deficiency of kidney essence，deficiency of
essence and bone，deficiency of essence and brain failure，deficiency of essence and lack of blood; Differentiation of shape:
shape of collaterals and internal shape of collaterals; Differentiation of mind: mental injury and depression，mental anxiety，
mental fatigue and forgetfulness，mental decline and insomnia． The physical and mental health status of sub-healthy and chro-
nic disease people was comprehensively analyzed and evaluated in four aspects of collaterals，essence，shape and spirit by u-
sing traditional Chinese medicine diagnostic methods and modern diagnostic techniques，providing the premise and basis for
the application of the“Eight-character theory of Tongluo Ｒegimen”to guide the implementation of health management．

This paper reported a case of autosomal dominant mental retardation type 22 caused by ZBTB18 gene muta-
tion，and reviewed literatures

The paper reported a case of nephrotic syndrome with syphilis and anticardiolipin antibody，and reviewed
the related literatures．

Neurodegenerative diseases are a kind of diseases characterized by neurodegenerative diseases． At present，
they occur frequently all over the world，and there is no effective treatment． Pulp stem cells of deciduous teeth are derived
from the pulp of deciduous teeth and from the neural crest． They have high neurogenic potential and can be used to treat a va-
riety of nervous system diseases． This article reviews the application and research progress of deciduous tooth pulp stem cells
( SHEDs) in tissue repair and regeneration of nervous system diseases．