目的 分析血清白介素-12(IL-12)、纤维蛋白原(Fib)水平预测新生儿坏死性小肠结肠炎(NEC)的价值。方法 选取2019年7月—2021年12月湖南中医药大学第一附属医院儿科收治的NEC患儿89例为NEC组，同时选取医院同龄健康新生儿85例为对照组。比较2组及不同分期NEC患儿血清IL-12、Fib水平的差异；Pearson分析评估NEC患儿血清IL-12、Fib水平与CRP、IL-6的相关性，ROC曲线评估血清IL-12、Fib水平对NEC的预测价值。结果 NEC组新生儿血清IL-12、Fib、CRP及IL-6水平均显著高于对照组(t/P=35.143/<0.001、22.466/<0.001、21.876/<0.001、22.157/<0.001);不同NEC分期患儿血清IL-12、Fib水平比较，Ⅲ期>Ⅱ期>Ⅰ期(F/P=10.329/<0.001、40.267/<0.001)。Pearson分析显示，血清IL-12、Fib水平与IL-6、CRP均呈正相关(IL-12:r/P=0.714/<0.001、0.679/<0.001;Fib:r/P=0.706/<0.001、0.652/<0.001)。ROC曲线分析显示，血清IL-12、Fib及二者联合诊断新生儿NEC的AUC分别为0.754、0.700、0.842,二者联合检测的价值明显高于单项指标(Z/P=6.154/<0.001、5.024/<0.001)。结论 新生儿NEC血清IL-12、Fib水平升高，二者联合检测对新生儿NEC具有良好的诊断效能，有利于临床早期提供针对性治疗。

目的 分析孕妇胎膜厚度、外周血辅助性T细胞17(Th17)/调节性T细胞(Treg)变化预测早产的价值。方法 选取2020年1月—2021年8月海南医学院第二附属医院妇产科就诊的先兆早产孕妇102例为观察组，再根据是否早产分为非早产亚组37例和早产亚组65例，另选取同期正常产检孕妇102例为对照组。比较2组孕妇胎膜厚度、外周血Th17/Treg水平及不同亚组间临床资料，采用Logistic回归模型分析早产的影响因素，ROC曲线分析胎膜厚度、外周血Th17/Treg水平预测早产的价值，并比较观察组新生儿中早产儿和足月儿并发症发生率。结果 观察组胎膜厚度、外周血Th17、Th17/Treg水平高于对照组，Treg水平低于对照组(t/P=3.976/<0.001,11.008/<0.001,39.875/<0.001,30.501/<0.001);早产亚组孕妇年龄≥30岁、孕周28～33周、妊娠期高血压疾病占比高于非早产亚组(χ2/P=6.585/0.010,4.184/0.041,3.972/0.046),胎膜厚度、Th17/Treg水平高于非早产亚组(t/P=2.837/0.006,3.210/0.002);胎膜厚度、Th17/Treg水平升高均是增加早产风险的独立危险因素[OR(95%CI)=3.683(1.869～7.257)、3.253(2.004～6.281)];胎膜厚度、Th17/Treg及二者联合预测早产的AUC为0.791、0.766、0.861,联合检测价值大于单项指标检测(Z/P=2.586/0.010,2.806/0.005);早产儿高胆红素血症、呼吸窘迫综合征发生率高于足月儿(χ2/P=6.585/0.010,4.184/0.041,3.972/0.046),胎膜厚度、Th17/Treg水平高于非早产亚组(t/P=2.837/0.006,3.210/0.002);胎膜厚度、Th17/Treg水平升高均是增加早产风险的独立危险因素[OR(95%CI)=3.683(1.869～7.257)、3.253(2.004～6.281)];胎膜厚度、Th17/Treg及二者联合预测早产的AUC为0.791、0.766、0.861,联合检测价值大于单项指标检测(Z/P=2.586/0.010,2.806/0.005);早产儿高胆红素血症、呼吸窘迫综合征发生率高于足月儿(χ2/P=4.067/0.044,8.074/0.005)。结论 孕妇胎膜厚度增加、外周血Th17/Treg水平升高时存在早产风险，检测二者水平对早产预测具有较高价值，有利于减少新生儿并发症。

目的 分析血清Sestrin2、抵抗素(Resistin)水平与妊娠晚期胎盘早剥(PA)及其预后的关系。方法 选取2018年9月—2021年12月于安徽医科大学附属六安医院分娩的妊娠晚期PA孕妇90例作为PA组，再根据妊娠结局分为不良预后亚组48例和良好预后亚组42例，并选取同期正常分娩的妊娠晚期孕妇90例作为正常分娩组。比较2组和PA不同妊娠结局亚组临床资料及血清Sestrin2、Resistin水平。Logistic回归分析妊娠晚期PA孕妇发生不良妊娠结局的影响因素；受试者工作特征曲线(ROC)评估血清Sestrin2、Resistin水平对妊娠晚期PA孕妇发生不良妊娠结局的预测价值。结果 PA组孕妇胎膜早破、妊娠期高血压疾病(HDCP)、妊娠期糖尿病(GDM)、机械损伤、羊水过多比例及血清Sestrin2、Resistin水平明显高于正常分娩组[χ2(t)/P=41.180/<0.001、4.808/0.028、4.367/0.037、6.197/0.013、4.655/0.031、12.230/<0.001、12.672/<0.001];不良预后亚组孕妇胎膜早破、HDCP、GDM、机械损伤、羊水过多比例及血清Sestrin2、Resistin水平明显高于良好预后亚组[χ2(t)/P=41.180/<0.001、4.808/0.028、4.367/0.037、6.197/0.013、4.655/0.031、12.230/<0.001、12.672/<0.001];不良预后亚组孕妇胎膜早破、HDCP、GDM、机械损伤、羊水过多比例及血清Sestrin2、Resistin水平明显高于良好预后亚组[χ2(t)/P=26.498/<0.001、5.727/0.017、6.509/0.011、4.533/0.033、6.494/0.011、3.358/0.001、5.355/<0.001]。Logistic回归分析结果表明胎膜早破、HDCP、GDM、机械损伤、羊水过多及血清Sestrin2高、血清Resistin高均是影响妊娠晚期PA孕妇发生不良妊娠结局的危险因素[OR(95%CI)=2.403(1.577～3.662)、2.691(1.711～4.232)、2.272(1.526～3.822)、2.586(1.670～4.004)、2.359(1.563～3.560)、2.983(1.831～4.860)、2.785(1.754～4.423)];血清Sestrin2、Resistin及两者联合预测妊娠晚期PA孕妇发生不良妊娠结局的曲线下面积(AUC)分别为0.869、0.866、0.950,两者联合预测的AUC高于单项预测(Z=2.410、2.499,P=0.016、0.012)。结论 妊娠晚期PA孕妇血清Sestrin2、Resistin水平较高，均与不良妊娠结局密切相关，两者联合可提高预测妊娠晚期PA孕妇妊娠结局不良的价值。

Objective To investigate the relationship between transvaginal color Doppler ultrasound parameters and pathological characteristics and prognosis of patients with endometrial cancer.Methods From January 2018 to January 2021, 197 patients with endometrial cancer(cancer group) and 75 patients with benign endometrial tumors(control group) who underwent transvaginal color Doppler ultrasonography in the Department of Gynecology of Northwest Women's and Children's Hospital were selected. Peak systolic velocity(PSV), pulsatility index(PI), and resistance index(RI) were measured. The differences of PSV, PI and RI among different pathological features were compared; Kaplan-Meier curves were drawn to analyze the difference of survival rate of endometrial cancer patients with different PSV, PI and RI levels. The COX hazard proportional regression model was used to analyze the prognostic factors of endometrial cancer patients.Results The PSV in the cancer group was higher than that in the control group, while the RI and PI were lower than those in the control group(t/P=14.846/<0.001, 4.254/<0.001, 11.862/<0.001). FIGO stage III-IV, muscle invasion ≥50% muscle layer, and lymph node metastasis patients with PSV were higher than FIGO stages I-II, muscle invasion degree <50% muscle layer, and no lymph node metastasis(t/P=9.718/<0.001, 10.216/<0.001, 8.291/<0.001), while RI and PI were lower than FIGO stages I-II, the degree of muscle invasion was less than 50% of the muscle layer, and there was no lymph node metastasis(RI:t/P=6.985/<0.001, 2.288/0.023, 5.162/<0.001; PI:t/P=7.797/<0.001, 3.940/<0.001, 3.872/<0.001). The overall survival time of endometrial cancer patients in high PSV subgroup was lower than that in low PSV subgroup(Z/P=5.326/<0.05), while the overall survival time of endometrial cancer patients in low RI and PI subgroups was lower than that in high RI and PI subgroups. Subgroup(Z/P=7.009/<0.05, 8.463/<0.05). FIGO stage III-IV, muscle invasion degree ≥50% of myometrium, lymph node metastasis, and elevated PSV are risk factors affecting the prognosis of patients with endometrial cancer [OR(95%CI)=1.865(1.292-2.690), 1.531(1.123-2.087), 1.919(1.294-3.846), 1.640(1.287-2.092)],and the increase of RI and PI were the protective factors [OR(95%CI)=0.876(0.611-0.981), 0.823(0.682-0.993)].Conclusion Transvaginal color Doppler ultrasonography can display the blood flow characteristics of endometrial cancer lesions. High PSV, low RI and low PI are related to the pathological characteristics and survival rate of endometrial cancer.

人口老龄化是我国今后较长一段时期的基本国情，人口老龄化伴随的多病寿短问题给社会经济带来了巨大压力。党的十九届五中全会将积极应对老龄化上升为国家战略，出台了一系列已老后应对方法。从中医治未病角度出发，以健康为中心的主动抗衰老是推进健康老龄化事业的关键措施。传承精华，守正创新，充分发挥中医药在抗衰老理论和实践方面的显著优势，全面发展中医药抗衰老事业具有高度的战略性、全局性、前瞻性，对于实现健康老龄化，建设健康中国具有重要意义。